Lipid-protein nanoscale bilayers: A versatile medium for NMR investigations of membrane proteins and membrane-active peptides
Lipid-protein nanoscale bilayers: A versatile medium for NMR investigations of membrane proteins and membrane-active peptides
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DOI：
10.1021/ja0777988
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发表时间：
2008-02-20
影响因子：
15
通讯作者：
Arseniev, Alexander S.
中科院分区：
文献类型：
--
作者：
Lyukmanova, Ekaterina N.;Shenkarev, Zakhar O.;Arseniev, Alexander S.
在目前的通信中，我们证明了使用高分辨率NMR在重组高密度脂蛋白（rHDL）颗粒膜蛋白的调查的可能性。rHDL颗粒是由载脂蛋白A-1的二聚体包裹的纳米级磷脂双层（Bayburt，T. H.的; Grinkova，Y.五、斯利加尔，S。G.纳米左。2002，2，853-856）。与常用的球形胶束相比，rHDL颗粒结合了脂质双层，如生物膜。这些颗粒仍然在NMR时间尺度上进行各向同性运动，提供了嵌入其双层中的肽和蛋白质的高分辨率NMR光谱的应用。作为一个例子，膜活性肽Antiamoebin-I在rHDL颗粒的双层中的拓扑结构通过使用脂溶性弛豫探针技术来确定。
In the present Communication we demonstrate the possibility to use high-resolution NMR for the investigation of membrane proteins in reconstituted high-density lipoprotein (rHDL) particles. The rHDL particles are nanoscale phospholipid bilayers wrapped around by a dimer of apolipoprotein A-1 (Bayburt, T. H.; Grinkova, Y. V.; Sligar, S. G. Nano Left. 2002, 2, 853-856). In contrast to the commonly used spherical micelles, the rHDL particles incorporate a lipid bilayer like in biological membranes. These particles still undergo isotropic motion on the NMR time scale, providing the application of high-resolution NMR spectroscopy of the peptides and proteins embedded into their bilayer. As an example, the topology of the membrane-active peptide Antiamoebin-I in the bilayer of the rHDL particles was determined by using the lipid-soluble relaxation probe technique.