Expression of a wheat MYB gene in transgenic tobacco enhances resistance to Ralstonia solanacearum, and to drought and salt stresses
Expression of a wheat MYB gene in transgenic tobacco enhances resistance to Ralstonia solanacearum, and to drought and salt stresses
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转基因烟草中小麦 MYB 基因的表达增强了对青枯菌、干旱和盐胁迫的抗性
DOI：
10.1007/s10142-011-0228-1
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发表时间：
2011-09-01
影响因子：
2.9
通讯作者：
Zhang, Zengyan
中科院分区：
文献类型：
--
作者：
Liu, Hongxia;Zhou, Xianyao;Zhang, Zengyan
MYB 转录因子在植物生长、发育过程和胁迫反应中发挥着不同的作用。从小麦 (Triticum aestivum L.) 中分离出 MYB 基因的全长 cDNA 序列，即 TaPIMP1。通过接种真菌病原体 Bipolaris sorokiniana 和干旱处理，TaPIMP1 转录水平显着上调。 TaPIMP1 编码由 323 个氨基酸组成的 MYB 蛋白 TaPIMP1。 TaPIMP1 包含两个 MYB DNA 结合域（R2、R3）、两个假定的核定位位点和两个假定的转录激活域。 TaPIMP1是R2R3-MYB转录因子亚家族的新成员。与TaPIMP1融合的GFP在洋葱表皮细胞中的瞬时表达证明TaPIMP1的亚细胞定位发生在细胞核中。通过农杆菌介导的转化将TaPIMP1基因转移到烟草(Nicotiana tabacum L.)品种W38中。通过 PCR 和 RT-PCR 分析进行筛选后，鉴定了表达 TaPIMP1 的转基因烟草品系，并评估了其病原体抗性、耐旱性和耐盐性。与未转化的烟草宿主植物相比，表达TaPIMP1的植物对青枯菌的抗性显着增强，并且对干旱和盐胁迫的耐受性得到改善。在这些转基因品系中，苯丙氨酸解氨酶（PAL）和超氧化物歧化酶（SOD）的活性相对于野生型烟草植物显着增加。
MYB transcription factors play diverse roles in plant growth, developmental processes and stress responses. A full-length cDNA sequence of a MYB gene, namely TaPIMP1, was isolated from wheat (Triticum aestivum L.). The TaPIMP1 transcript level was significantly up-regulated by inoculation with a fungal pathogen Bipolaris sorokiniana and by drought treatment. TaPIMP1 encodes the MYB protein TaPIMP1 consisting of 323 amino acids. TaPIMP1 contains two MYB DNA binding domains (R2, R3), two putative nuclear localization sites and two putative transcription activation domains. TaPIMP1 is a new member of the R2R3-MYB transcription factor subfamily. Transient expression in onion epidermal cells of GFP fused with TaPIMP1 proved that subcellular localization of TaPIMP1 occurred in the nucleus. The TaPIMP1 gene was transferred into tobacco (Nicotiana tabacum L.) cultivar W38 by Agrobacterium-mediated transformation. After screening through PCR and RT–PCR analyses, transgenic tobacco lines expressing TaPIMP1 were identified and evaluated for pathogen resistance, and drought and salt tolerance. Compared to untransformed tobacco host plants, TaPIMP1 expressing plants displayed significantly enhanced resistance to Ralstonia solanacearum and exhibited improved tolerances to drought and salt stresses. In these transgenic lines, the activities of phenylalanine ammonia-lyase (PAL) and superoxide dismutase (SOD) were significantly increased relative to wild-type tobacco plants.