Genes involved in formation of structured multicellular communities by Bacillus subtilis
Genes involved in formation of structured multicellular communities by Bacillus subtilis
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DOI：
10.1128/jb.186.12.3970-3979.2004
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发表时间：
2004-06-01
影响因子：
3.2
通讯作者：
Kolter, R
中科院分区：
文献类型：
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作者：
Branda, SS;González-Pastor, JE;Kolter, R
芽胞形成细菌枯草芽孢杆菌能够组装多细胞群落(生物膜)，表现出高度的时空组织性。未在实验室驯化的野生菌株产生特别强大的生物膜，具有复杂的结构特征，例如子实体状的空中突起，其尖端是产孢子的优先地点。为了发现参与这种多细胞行为的基因，并在全基因组的基础上这样做，我们利用了大量突变体的优势，这些突变体扰乱了枯草杆菌基因组中大多数未表征的基因。这个收集是用实验室菌株产生的，对在生物膜形成中受损的突变进行了筛选。然后将这一突变基因亚集导入野生菌NCIB 3610中，研究它们对液体和固体介质中生物纤维形成的影响。通过这种方式，我们确定了参与多细胞群落发展的六个基因。它们分别是yhxB(编码一个可能参与胞外多糖合成的磷酸六聚酶)、sipW(编码一个信号肽)、ECSB(编码一个ABC转运子亚基)、yqeK(编码一个可能的磷酸酶)、ylbF(编码一个调节蛋白)和ymca(一个功能未知的基因)。进一步分析表明，这6个基因在枯草芽孢杆菌群落发育过程中起着不同的作用。
The spore-forming bacterium Bacillus subtilis is capable of assembling multicellular communities (biofilms) that display a high degree of spatiotemporal organization. Wild strains that have not undergone domestication in the laboratory produce particularly robust biofilms with complex architectural features, such as fruiting-body-like aerial projections whose tips serve as preferential sites for sporulation. To discover genes involved in this multicellular behavior and to do so on a genome-wide basis, we took advantage of a large collection of mutants which have disruptions of most of the uncharacterized genes in the B. subtilis genome. This collection, which was generated with a laboratory strain, was screened for mutants that were impaired in biofilm formation. This subset of mutated genes was then introduced into the wild strain NCIB 3610 to study their effects on biofihn formation in liquid and solid media. In this way we identified six genes that are involved in the development of multicellular communities. These are yhxB (encoding a putative phosphohexomutase that may mediate exopolysaccharide synthesis), sipW (encoding a signal peptidase), ecsB (encoding an ABC transporter subunit), yqeK (encoding a putative phosphatase), ylbF (encoding a regulatory protein), and ymcA (a gene of unknown function). Further analysis revealed that these six genes play different roles in B. subtilis community development.