Mitochondrial transfer from aged adipose-derived stem cells does not improve the quality of aged oocytes in C57BL/6 mice
Mitochondrial transfer from aged adipose-derived stem cells does not improve the quality of aged oocytes in C57BL/6 mice
复制标题
来自衰老脂肪干细胞的线粒体移植并不能改善 C57BL/6 小鼠衰老卵母细胞的质量
DOI：
10.1002/mrd.23129
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发表时间：
2019-05-01
影响因子：
2.5
通讯作者：
Sun, Haixiang
中科院分区：
文献类型：
--
作者：
Sheng, Xiaoqiang;Yang, Yanjun;Sun, Haixiang
女性生育能力在35岁以上急剧下降，这是由于年龄相关的卵母细胞质量和数量下降。虽然线粒体移植有望成为一种可以改善这种年龄受损的卵母细胞质量的技术，但理想的线粒体供体仍然难以捉摸。在本研究中，我们的目的是确定老年脂肪来源的干细胞是否构成一个优秀的线粒体供体，将提高老年小鼠卵母细胞的质量。我们发现，衰老显著损害小鼠卵母细胞的线粒体功能，但并没有显着影响脂肪来源的干细胞的线粒体功能。然而，从老年脂肪干细胞的线粒体转移并没有减轻老年卵母细胞的受精率和胚胎发育率。
Female fertility declines dramatically over the age of 35 due to age-related decreases in oocyte quality and quantity. Although mitochondrial transfer promises to be a technology that can improve the quality of such age-impaired oocytes, the ideal mitochondrial donor remains elusive. In the present study, we aimed to identify whether aged adipose-derived stem cells constitute an excellent mitochondrial donor that would improve the quality of aged mouse oocytes. We showed that aging significantly impaired the mitochondrial function in mouse oocytes, but did not significantly affect the mitochondrial function of adipose-derived stem cells. However, the mitochondrial transfer from aged adipose-derived stem cells did not mitigate the poor fertilization and embryonic development rates of aged oocytes.