Antibiotics-free stable polyhydroxyalkanoate (PHA) production from carbon dioxide by recombinant cyanobacteria
Antibiotics-free stable polyhydroxyalkanoate (PHA) production from carbon dioxide by recombinant cyanobacteria
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DOI：
10.1016/j.biortech.2011.09.058
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发表时间：
2011-12-01
影响因子：
11.4
通讯作者：
Miyasaka, Hitoshi
中科院分区：
文献类型：
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作者：
Akiyama, Hideo;Okuhata, Hiroshi;Miyasaka, Hitoshi
利用蓝藻recA无效突变与大肠杆菌recA基因互补的原理，建立了一种实用的蓝藻无菌质粒表达系统。该系统被应用于生产聚羟基烷酸酯（PHA），一种可生物降解的塑料，和转基因蓝藻稳定地保持PHA生产的PHA基因在无菌培养基中，并积累高达52%的细胞干重的PHA。(C)2011爱思唯尔有限公司保留所有权利。
A practical antibiotics-free plasmid expression system in cyanobacteria was developed by using the complementation of cyanobacterial recA null mutation with the Escherichia coli recA gene on the plasmid. This system was applied to the production of polyhydroxyalkanoate (PHA), a biodegradable plastic, and the transgenic cyanobacteria stably maintained the pha genes for PHA production in the antibiotics-free medium, and accumulated up to 52% cell dry weight of PHA. (C) 2011 Elsevier Ltd. All rights reserved.