Determination of psilocin, bufotenine, LSD and its metabolites in serum, plasma and urine by SPE-LC-MS/MS
Determination of psilocin, bufotenine, LSD and its metabolites in serum, plasma and urine by SPE-LC-MS/MS
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DOI：
10.1007/s00414-012-0796-1
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发表时间：
2013-05-01
影响因子：
2.1
通讯作者：
Koehler, Helga
中科院分区：
文献类型：
--
作者：
Martin, Rafaela;Schuerenkamp, Jennifer;Koehler, Helga
建立了同时测定血清、血浆和尿液中裸盖菇素、蟾毒替宁、麦角酰二乙胺及其代谢产物的液相色谱-电喷雾串联质谱分析方法。在用聚合物混合模式阳离子交换柱进行固相萃取过程中，不稳定的分析物用抗坏血酸保护，用氮气干燥并避光。所有分析物的检测限和定量限均较低。所有分析物的回收率均为千分之86%，未观察到显著的基质效应。不同浓度下的日间和日内不精密度范围为1.1 - 8.2%相对标准偏差，偏倚在+/-5.3%范围内。处理后的样品在自动进样器中可稳定至少2天。此外，还研究了冻融和长期稳定性。该方法已成功应用于真实的血清和尿液样品。
A validated method for the simultaneous determination of psilocin, bufotenine, lysergic acid diethylamide and its metabolites in serum, plasma and urine using liquid chromatography-electrospray ionization/tandem mass spectrometry was developed. During the solid-phase extraction procedure with polymeric mixed-mode cation exchange columns, the unstable analytes were protected by ascorbic acid, drying with nitrogen and exclusion of light. The limits of detection and quantitation for all analytes were low. Recovery was a parts per thousand yen86 % for all analytes and no significant matrix effects were observed. Interday and intraday imprecisions at different concentrations ranged from 1.1 to 8.2 % relative standard deviation, bias was within +/- 5.3 %. Processed samples were stable in the autosampler for at least 2 days. Furthermore, freeze/thaw and long-term stability were investigated. The method was successfully applied to authentic serum and urine samples.