Gastroprotective effect of standardized leaf extract from Argyreia speciosa on experimental gastric ulcers in rats
Gastroprotective effect of standardized leaf extract from Argyreia speciosa on experimental gastric ulcers in rats
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DOI：
10.1016/j.jep.2011.05.028
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发表时间：
2011-09-01
影响因子：
5.4
通讯作者：
Dubey, Mukesh K.
中科院分区：
文献类型：
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作者：
Jaiswal, Sunil K.;Rao, Chandana V.;Dubey, Mukesh K.
民族药理学相关性：白银（L.f），甜（旋花科）在印度医学体系中传统上用作春药、返老还童剂、智力促进剂、健脑剂以及治疗肝肿大、糖尿病和慢性溃疡。研究目的：研究白银叶标准化丁醇部分（ASE）的胃保护作用。材料和方法：口服大花银叶的丁醇部分（ASE；50、100 和 200 毫克/千克体重），每天两次，持续 5 天，以预防阿司匹林 (ASP)、乙醇 (EtOH)、冷约束应激 (CRS) 和幽门结扎 (PL) 诱发的溃疡。在CRS诱导的溃疡模型中进行抗氧化酶活性的估计，并在PL诱导的溃疡模型中估计各种胃分泌参数，如胃液量、胃酸排出量和pH值。 结果：ASE在ASP 23.64-58.76%（p < 0.01至p < 0.001）中表现出剂量依赖性溃疡保护作用。 EtOH 15.45-58.45% (p < 0.001)，CRS 19.39-78.36% (p < 0.001) 和 PL 19.67-69.04% (p < 0.05 至 p < 0.01)。分别。在各种胃溃疡模型中，标准药物雷尼替丁的保护率为 77.77-84.32%（p < 0.01 至 p < 0.001）。 ASE 显着 (p < 0.001) 增强胃壁粘液，被视为对抗 EtOH 诱导的胃溃疡的第一道防线，显示出细胞保护特性。 ASE 显示体积、酸性胃蛋白酶浓度和酸性胃蛋白酶输出略有下降。然而，与 CRS 诱导组相比，ASE 降低了溃疡指数，LPO 水平（p < 0.001）和 SOD 水平（p < 0.01 至 p < 0.001）显着降低。在 100 和 200 mg/kg 剂量水平下，观察到 CAT 值逐渐显着增加（p < 0.01 至 p < 0.001）。 结论：我们的研究结果表明，大花银具有显着的剂量依赖性胃保护活性，这可能是由于其自由基清除活性。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: Argyreia speciosa (L.f), Sweet (Family Convolvulaceae) is used traditionally in Indian System of Medicine as aphrodisiac, rejuvenating agent, intellect promoting agent, brain tonic and in the therapy of hepatomegaly, diabetes and chronic ulcer.Aim of the study: To study the gastroprotective effect of standardized butanol fraction of Argyreia speciosa leaf (ASE).Materials and methods: The butanol fraction of Argyreia speciosa leaf (ASE; 50, 100 and 200 mg/kg body weight)was administered orally, twice daily for 5 days for prevention from Aspirin (ASP)-, ethanol (EtOH)-, cold-restraint stress (CRS) - and pylorus ligation (PL)-induced ulcers. Estimation of antioxidant enzymes activity was carried out in CRS-induced ulcer model, and various gastric secretion parameters like volume of gastric juice, acid output, and pH value were estimated in PL-induced ulcer model.Result: ASE showed dose-dependent ulcer protective effect in ASP 23.64-58.76% (p < 0.01 to p < 0.001). EtOH 15.45-58.45% (p < 0.001), CRS 19.39-78.36% (p < 0.001) and PL 19.67-69.04% (p < 0.05 to p < 0.01). respectively. The percentage of protection by standard drug ranitidine was 77.77-84.32% (p < 0.01 to p < 0.001) in various gastric ulcer models. The gastric wall mucus was significantly (p < 0.001) enhanced by ASE and is regarded as the first line of defence against EtOH-induced gastric ulcers showing cytoprotective property. ASE showed a marginal decrease in volume, acid pepsin concentration and acid pepsin output. However, ASE reduced the ulcer index with significant decrease in LPO level (p < 0.001), and SOD level (p < 0.01 to p < 0.001) as compared with CRS-induced group. A gradual and significant increase in CAT values were observed at 100 and 200 mg/kg dose levels (p < 0.01 to p < 0.001).Conclusions: The results of our study revealed that Argyreia speciosa possess significant dose dependent gastroprotective activity, probably due to its free radical scavenging activity. (C) 2011 Elsevier Ireland Ltd. All rights reserved.