Comparison of the primary structure ofwaxy proteins (granule-bound starch synthase) between polyploid wheats and related diploid species
Comparison of the primary structure ofwaxy proteins (granule-bound starch synthase) between polyploid wheats and related diploid species
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多倍体小麦与相关二倍体物种蜡质蛋白（颗粒结合淀粉合酶）一级结构的比较
DOI：
10.1007/bf00570121
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发表时间：
1996
影响因子：
2.4
通讯作者：
T. Taira
中科院分区：
文献类型：
--
作者：
N. Fujita;A. Wadano;S. Kozaki;K. Takaoka;S. Okabe;T. Taira
利用SDS-PAGE对多倍体小麦及其相关二倍体物种基因组A、B、D编码的蜡蛋白进行了分离。测定了成熟蛋白和V8蛋白诱导片段的N末端氨基酸序列。在这些序列中共检测到5个氨基酸替换，约占蜡质蛋白全序列的10%。多倍体小麦与相关二倍体物种的这些序列的比较表明：(1)多倍体小麦A基因组编码的蜡蛋白与单粒小麦完全相同；(2)B基因组编码的蜡蛋白。浮肿和平时的情况完全一样。Searsii，但与通常的不同。拼写和T。(3)由B基因组编码的蜡蛋白。Aestivum不同于通常由B基因组编码的Aestivum。(4)D基因组编码的蜡蛋白。平均水平和通常情况下是一样的。陶斯奇。
Thewaxyproteins encoded by the genomes A, B, and D in polyploid wheats and related diploid species were isolated by SDS-PAGE. The N-terminal amino acid sequences of mature proteins and V8 protease-induced fragments were determined. A total of five amino acid substitutions was detected in these sequences, which represent about 10% of the whole sequences of thewaxyproteins. A comparison of these sequences in polyploid wheats with those in related diploid species revealed the following: (i)waxyproteins encoded by the A genome of polyploid wheats were identical to that ofTriticum monococcum, (ii) thewaxyprotein encoded by the B genome ofT. turgidumwas identical to that ofT. searsii, but differed from those ofT. speltoidesandT. longissimumby one amino acid substitution, (iii) thewaxyprotein encoded by the B genome ofT. aestivumdiffered from that encoded by the B genome ofT. turgidumby one amino acid substitution, and (iv) thewaxyprotein encoded by the D genome ofT. aestivumwas identical to that ofT. tauschii.