Dynamic variations of local cerebral blood flow in maximal electroshock seizures in the rat
Dynamic variations of local cerebral blood flow in maximal electroshock seizures in the rat
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DOI：
10.1046/j.1528-1157.2002.17702.x
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发表时间：
2002-10-01
期刊：
影响因子：
5.6
通讯作者：
Nehlig, A
中科院分区：
文献类型：
--
作者：
André, V;Henry, D;Nehlig, A
目的：测量脑血流量是常规用于定位癫痫患者癫痫发作发生和扩散的区域。由于高灌注区性质随示踪剂注射时间的变化而变化，在本研究中，我们使用最大电休克发作大鼠模型来随访癫痫引起的脑血流（CBF）变化分布的时间依赖性变化。方法：采用定量放射自显影[C-14]碘安替比林技术测定CBF，持续时间30 s。示踪剂可在癫痫诱导前15秒注射，同时注射电击（强直期），在阵挛期开始时注射，或在癫痫发作后3和6分钟注射（阳性期）。结果：脑血流率在癫痫发作活动的不同阶段发生动态变化，在癫痫发作的所有阶段（前3次）研究的45个区域中，脑血流率大大高于对照水平（小于或等于400%）。在癫痫发作后3分钟和6分钟，35个和15个区域的CBF分别高于对照水平。结论：最大CBF增加的分布与其已知的参与不同类型癫痫发作活动（强直性和阵挛性）临床表达的电路有很好的相关性。
Purpose: Measurement of cerebral blood flow is routinely used to locate the areas involved in generation and spread of seizures in epilepsy patients. Because the nature of the hyperperfused regions varies with the timing of injection of tracer, in this study, we used a rat model of maximal electroshock seizures to follow up the time-dependent changes in the distribution of seizure-induced cerebral blood flow (CBF) changes.Methods: CBF was measured by the quantitative autoradiographic [C-14] iodoantipyrine technique over a 30-s duration. The tracer was injected either at 15 s before seizure induction, simultaneous with the application of the electroshock (tonic phase), at the onset of the clonic phase, or at 3 and 6 min after the seizure (postictal phase).Results: Rates of CBF underwent dynamic changes during the different phases of seizure activity and largely increased over control levels (less than or equal to400%) in the 45 regions studied during all phases of the seizure (first 3 times). CBF remained higher than control levels in 35 and 15 areas at 3 and 6 min after the seizure, respectively.Conclusions: The distribution of maximal CBF increases showed a good correlation with their known involvement in the circuits underlying the clinical expression of the different types of seizure activity, tonic versus clonic.