Establishment of a small animal model for human Helicobacter pylori infection using germ-free mouse.
Establishment of a small animal model for human Helicobacter pylori infection using germ-free mouse.
复制标题
使用无菌小鼠建立人幽门螺杆菌感染小动物模型。
DOI：
--
复制
发表时间：
1994
影响因子：
9.8
通讯作者：
Kiwamu Okita
中科院分区：
文献类型：
--
作者：
M. Karita;Qian Li;Daniel Cantero;Kiwamu Okita
目的
了解为什么幽门螺杆菌口服接种导致无菌无胸腺小鼠胃中持续定植，但在非无菌小鼠中仅暂时定植。
方法
我们用H. pylori，并研究了由此产生的胃定植，将其与无菌无胸腺小鼠模型进行比较。此外，我们研究了上述三个小鼠组中的乳酸杆菌。
结果
H. pylori在所有无菌无胸腺小鼠和所有无菌正常胸腺小鼠中连续检测。然而，在所有非无菌的正常胸腺小鼠中，H。pylori仅在接种后暂时检测到。仅在非无菌小鼠组中检测到乳酸杆菌。H.在接种后的研究期间，无菌正常胸腺小鼠中的pylori显著低于无菌无胸腺小鼠。
结论
因此，我们认为H.幽门可以被免疫系统抑制，并被先前栖息在胃中的乳酸杆菌根除。
OBJECTIVE
To understand why oral inoculation of Helicobacter pylori resulted in continuous colonization of the stomach in germ-free athymic mice, but only temporary colonization in mice that were not germ-free.
METHODS
We inoculated germ-free and "not-germ-free" euthymic mice with H. pylori and studied the resulting colonization of the stomach, comparing it against the germ-free athymic mouse model. In addition, we investigated Lactobacillus in the above-described three mouse groups.
RESULTS
H. pylori were detected in all germ-free athymic mice and all germ-free euthymic mice continuously. However, in all euthymic mice that were not germ-free, H. pylori was detected only temporarily after inoculation. Lactobacilli were detected only in the not-germ-free mouse group. The number of H. pylori in the germ-free euthymic mice was significantly lower than in the germ-free athymic mice during the period of this study after inoculation.
CONCLUSIONS
We therefore suggest that the growth of H. pylori may be suppressed by the immunological system and eradicated by Lactobacilli previously inhabiting the stomach.