Rapid diagnosis of M-tuberculosis using a piezoelectric immunosensor
Rapid diagnosis of M-tuberculosis using a piezoelectric immunosensor
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DOI：
10.2116/analsci.18.397
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发表时间：
2002-04-01
影响因子：
1.6
通讯作者：
Zhang, LD
中科院分区：
文献类型：
--
作者：
He, FJ;Zhang, LD
开发了一种压电免疫传感器用于检测结核分枝杆菌（M. TB）。将蛋白质 A 修饰到镀银压电石英晶体传感器上。抗体可以通过蛋白A以有序的方向键合到晶体表面上。使用阻抗分析仪来记录导纳-频率曲线。实验结果表明 Protein A 和抗体成功且不太刚性地结合。与其他传统结核病检测方法相比，本文提出的方法灵敏、选择性强、有效。使用对照抗体-兔抗蜂毒（抗-HBV）限制了非特异性反应。使用甘氨酸-HCl 缓冲溶液（pH = 2.4）从涂有蛋白 A 和 0.5 mg/mL 抗结核菌的晶体中释放抗体；用于从由抗体结合的晶体中去除结核病。表现出良好的可重复使用性。使用所提出的方法对添加或不添加结核分枝杆菌的正常人的痰液和唾液的尖峰样本进行诊断。取得了良好的效果。
A piezoelectric immunosensor was developed for detecting Mycobacteria Tuberculosis (M. TB). Protein A was modified onto a silver-coated piezoelectric quartz-crystal sensor. Antibodies could be bond onto the crystal surface in an ordered orientation through protein A. An impendence analyzer was employed to record the admittance-frequency curve. The experimental results showed a successful and less-rigid bonding of protein A and antibodies. Compared with other traditional detection methods of TB, the method suggested in this paper was sensitive, selective and effective. The nonspecific response was limited by using control antibody-rabbit anti-honey bee venom (anti-HBV). A glycine-HCl buffer solution (pH = 2.4) was used to release antibodies from a crystal coated with protein A, and 0.5 mg/mL anti-TB; was used to remove TB from a crystal that was bonded by an antibody. Good reusability was exhibited. Spike samples of sputum and saliva from normal people with and without adding M. tuberculosis were diagnosed using the proposed method. Good results were obtained.