In vivo kinetic study of materno-fetal fatty acid transfer in obese and normal weight pregnant women
In vivo kinetic study of materno-fetal fatty acid transfer in obese and normal weight pregnant women
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DOI：
10.1113/jp278146
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发表时间：
2019-07-24
影响因子：
5.5
通讯作者：
Larque, Elvira
中科院分区：
文献类型：
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作者：
Gazquez, Antonio;Prieto-Sanchez, Maria T.;Larque, Elvira
我们首次分析了对照和肥胖（OB）孕妇中稳定同位素标记的脂肪酸（FA）的体内母胎动态转移。分别在择期剖腹产前-4 h、-8 h和-12 h对10名体重指数>30（OB）和10名体重指数在20-25（NW）范围内的孕妇口服具有相似代谢的标记FA（硬脂酸：C-13-SA;棕榈酸：C-13-PA;油酸：C-13-OA）。收集胎盘、静脉和动脉脐带血，获得广泛的FA富集。一个相结合的实验和计算建模分析。胎盘中FA合成率（FSR）为11- 12%h（-1）。NW和正常血流量OB之间没有观察到差异。使用这种口服推注剂量方法无法估计脐带血中的FA FSR。计算建模表明，当包括所有母体血浆脂质类别时，计算建模与数据拟合良好，但仅基于非酯化FA组分的建模并非如此。估计的母胎C-13-FA转移相似于1%。总之，我们使用多种C-13-FA示踪剂的方法使我们能够估计胎盘/母体血浆中的FSR，但不能估计胎儿/母体隔室中的FSR。计算模型显示胎盘C-13-FA转移的一致时间过程，并预测在实验期间胎儿总FA积累。我们的结论是，除了非酯化FA馏分在母体循环中，母体血浆极低密度脂蛋白和其他脂蛋白是重要的贡献者胎盘FA转移到胎儿。
We analyse for the first time the in vivo materno-fetal kinetic transfer of fatty acids (FA) labelled with stable isotopes in control and obese (OB) pregnant women. Labelled FA with a similar metabolism (stearic acid: C-13-SA; palmitic acid: C-13-PA; oleic acid: C-13-OA) were orally administered at -4 h, -8 h and -12 h, respectively prior to elective caesarean section to 10 pregnant women with a body mass index >30 (OB) and 10 with a body mass index in the range 20-25 (NW). Placenta, venous and arterial cord blood were collected obtaining a wide range of FA enrichments. A combined experimental and computational modelling analysis was applied. FA fractional synthesis rate (FSR) in placenta was 11-12% h(-1). No differences were observed between NW and normo-lipidemic OB. It was not possible to estimate FA FSR in cord blood with this oral bolus dose approach. Computational modelling demonstrated a good fit to the data when all maternal plasma lipid classes were included but not with modelling based only on the non-esterified FA fraction. The estimated materno-fetal C-13-FA transfer was similar to 1%. In conclusion, our approach using multiple C-13-FA tracers allowed us to estimated FSR in placental/maternal plasma but not in fetal/maternal compartments. Computational modelling showed a consistent time course of placental C-13-FA transfer and predicted total fetal FA accumulation during the experiment. We conclude that, in addition to non-esterified FA fraction in the maternal circulation, maternal plasma very low-density lipoprotein and other lipoproteins are important contributors to placental FA transfer to the fetus.