Effect of Staphylococcus aureus extracellular proteinaceous fraction in an isolated osteoclastic resorption assay
Effect of Staphylococcus aureus extracellular proteinaceous fraction in an isolated osteoclastic resorption assay
复制标题
金黄色葡萄球菌胞外蛋白质组分在分离破骨细胞吸收测定中的作用
DOI：
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发表时间：
1998
影响因子：
3.3
通讯作者：
A. Boyde
中科院分区：
文献类型：
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作者：
M. Arora;N. Shah;Sajeda Meghji;Brian E. Henderson;Malcolm Harris;Sean P. Nair;Michael Wilson;C. Gray;Sheila J. Jones;A. Boyde
翻译后摘要：金黄色葡萄球菌感染的骨引起严重的骨损伤，但负责的机制仍有待建立。我们使用由金黄色葡萄球菌（SAM）的盐水提取物释放的富含蛋白质的部分来测试表面相关蛋白通过直接激活破骨细胞促进骨分解的假设。分离的小鸡破骨细胞在有或没有S.金黄色的使用共聚焦反射显微镜表面测绘测量所得的骨细胞挖掘。SAM既刺激了再吸收坑的形成，又诱导了具有较大体积面积比的坑的产生。
Abstract: Staphylococcus aureus infection of bone causes severe bone damage but the mechanisms responsible remain to be established. We used the protein-rich fraction released by saline extraction of Staphylococcus aureus (SAM) to test the hypothesis that the surface-associated proteins promote bone breakdown by directly activating osteoclasts. Isolated chick osteoclasts were incubated on dentine slices with or without the surface protein fraction from S. aureus. The resulting osteoclastic excavations were measured using confocal reflection microscopic surface mapping. SAM both stimulated the formation of resorption pits and induced the production of pits with larger volume : area ratios..