Analytical methodology for TEX86 paleothermometry by high-performance liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry.
Analytical methodology for TEX86 paleothermometry by high-performance liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry.
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DOI：
10.1021/ac062339v
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发表时间：
2007-02
影响因子：
7.4
通讯作者：
Stefan Schouten;C. Huguet;E. Hopmans;M. Kienhuis;J. Damsté
中科院分区：
文献类型：
--
作者：
Stefan Schouten;C. Huguet;E. Hopmans;M. Kienhuis;J. Damsté
TEX 86是最近提出的一种古温度计，通过它可以重建120万年前的古海水温度。其基于通过高效液相色谱法/大气压化学电离-质谱法（HPLC/APCI-MS）测定的甘油二植烷基甘油四醚的相对分布。本研究的目的是检查和改进几个分析方面的确定这一重要的代理环境矩阵。分别使用单离子模式（SIM）和质量扫描（m/z 950至1450）检测的TEX 86分析的比较显示，SIM的灵敏度高达2个数量级，并且使用该方法可以以+/-0.004或+/-0.3 ℃的再现性测定TEX 86。用两种不同的HPLC/APCI-MS设置获得的TEX 86值的比较显示没有显著差异。此外，通过索氏提取、超声波和加速高压提取技术获得的提取物的TEX 86分析也表明，两种方法之间没有显著差异。我们的研究结果表明，TEX 86的HPLC/APCI-MS分析是强大的，可以确定与其他温度代理的分析误差相当。
The TEX86 is a recently proposed paleothermometer through which ancient seawater temperatures of up to 120 My ago can be reconstructed. It is based on the relative distribution of glycerol dibiphytanyl glycerol tetraethers as measured by high-performance liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS). The aim of this study was to examine and improve several analytical aspects in the determination of this important proxy in environmental matrices. Comparison of TEX86 analysis using single ion mode (SIM) and mass scanning (m/z 950 to 1450) detection, respectively, revealed that SIM is up to 2 orders of magnitude more sensitive and that the TEX86 can be determined with a reproducibility of +/-0.004 or +/-0.3 degrees C using this method. Comparison of TEX86 values obtained with two different HPLC/APCI-MS set-ups revealed no significant differences. In addition, analysis of TEX86 of extracts obtained by Soxhlet, ultrasonic, and accelerated high-pressure extraction techniques also showed no significant differences between the methods. Our results suggest that TEX86 analysis by HPLC/APCI-MS is robust and can be determined with analytical errors comparable to those of other temperature proxies.