Hormonal regulation of hormone-sensitive lipase activity and mRNA levels in isolated rat adipocytes.
Hormonal regulation of hormone-sensitive lipase activity and mRNA levels in isolated rat adipocytes.
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发表时间：
1994-09
影响因子：
6.5
通讯作者：
B. Slavin;J. Ong;P. Kern
中科院分区：
文献类型：
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作者：
B. Slavin;J. Ong;P. Kern
激素敏感脂肪酶(HSL)介导哺乳动物脂肪细胞三酰甘油的脂解，导致非酯化脂肪酸和甘油的释放。虽然许多研究已经研究了HSL的激素调节，但对激素调节反应的HSL mRNA水平的测量还没有研究。本研究旨在通过测定原代培养的大鼠脂肪细胞HSL mRNA水平和甘油释放来确定肾上腺素、生长激素、胰高血糖素和地塞米松对HSL表达的影响。脂肪细胞暴露于10(-7)M和10(-5)M肾上腺素4h后，中甘油含量增加(分别为对照的2 0 9+/-46%和2 84+/-5 8%，P<；0.001)。然而，HSL mRNA水平并未因肾上腺素治疗而改变。同样，胰升糖素(10(-7)M和10(-5)M，作用4h)和生长激素(100 ng/ml，作用24 h)可使脂肪细胞中甘油含量升高，但对HSL基因表达水平无影响。在脂肪细胞培养中加入地塞米松4小时和24小时，中甘油含量呈剂量依赖性增加(10~(-8)M、10~(-7)M和10~(-6)M分别为102.0%、138.8%(P<；0.001)和168.24%(P<；0.001)。
Hormone-sensitive lipase (HSL) mediates the lipolysis of triacylglycerol from mammalian adipocytes, resulting in the release of non-esterified fatty acids and glycerol. Although numerous studies have examined the hormonal regulation of HSL, the measurement of HSL mRNA levels in response to hormonal regulators has not been studied. This study was designed to determine the effects of epinephrine, growth hormone, glucagon, and dexamethasone on HSL expression by measuring HSL mRNA levels and glycerol release in primary cultures of rat adipocytes. Exposure of adipocytes to epinephrine at 10(-7) M and 10(-5) M for 4 h resulted in an increase in medium glycerol (209 +/- 46%, and 284 +/- 58% of control, P < 0.001, respectively). However, no change in HSL mRNA levels occurred due to the epinephrine treatment. Similarly, the peptides glucagon (10(-7) M and 10(-5) M for 4 h) and growth hormone (100 ng/ml for 24 h) resulted in increased medium glycerol and had no effect on HSL mRNA levels in adipocytes. Dexamethasone was added to adipocyte cultures for 4 and 24 h, and resulted in a dose-dependent increase of medium glycerol (102 +/- 8%, 138 +/- 8% (P < 0.001), and 168 +/- 24% (P < 0.001) for 10(-8) M, 10(-7) M, and 10(-6) M, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)