Fatty acid content in muscles of amago salmon homozygous or heterozygous for a growth hormone transgene.
Fatty acid content in muscles of amago salmon homozygous or heterozygous for a growth hormone transgene.
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生长激素转基因纯合子或杂合子鲑鱼肌肉中的脂肪酸含量。
DOI：
10.1016/j.aquaculture.2014.10.020
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发表时间：
2015
期刊：
影响因子：
4.5
通讯作者：
and Tsukasa Mori.
中科院分区：
文献类型：
--
作者：
Manabu Sugiyama;Fumio Takenaga;Hiroyuki Okamoto;Tetsuji Masaoka;Kazuo Araki;Hiroyuki Nagoya;and Tsukasa Mori.
脂肪酸是鱼类的重要能量来源，对鱼类的生理健康具有重要意义。转基因生长激素（GH）的Amago salmon （Oncorhynchus masouishikawae）表现出生长加速和肝脏组织脂肪酸组成和含量的改变。特别是饱和脂肪酸和单不饱和脂肪酸减少，多不饱和脂肪酸增加，但二十二碳六烯酸（22:6n - 3）和二十碳五烯酸（20:5n - 3）除外。此外，转基因鱼的血清葡萄糖、甘油三酯水平降低，3-羟基丁酸水平升高，3-羟基丁酸通常被认为是饥饿的标志。由于肝组织与肌肉组织在生理上是相连的，我们在这里研究了生长激素转基因对纯合子和杂合子转基因生长激素鱼肌肉中脂肪酸含量的影响。主要单不饱和脂肪酸油酸（18:1n−9）和棕榈油酸（16:1n−7）在对照组中略高，而多不饱和脂肪酸（22:6n−3和20:5n−3除外）在转基因鱼中显著高于对照组（P< 0.05），与肝脏结果相似。然而，与肝脏相比，转基因鱼的主要饱和脂肪酸棕榈酸（16:0）和硬脂酸（18:0）以及多不饱和脂肪酸（22:6n−3）和（20:5n−3）显著高于对照组（P< 0.05）。
Fatty acids are a vital energy source in fish and are of significant importance to their physiological wellbeing. Amago salmon (Oncorhynchus masouishikawae) transgenic for a growth hormone (GH) show both accelerated growth and altered fatty acid composition and content in liver tissues. In particular, they show a decrease in saturated fatty acids and monounsaturated fatty acids, and an increase in polyunsaturated fatty acids except for docosahexaenoic acid (22:6n− 3) and eicosapentaenoic acid (20:5n − 3). Furthermore, transgenic fish have decreased levels of serum glucose, triacylglycerol and an increase in 3-hydroxybutyric acid, generally considered a starvation marker. As liver tissue is physiologically connected to muscle tissue, here we examined the effects of GH transgenesis on fatty acid contents in muscles of homozygous and heterozygous GH transgenic fish. The major monounsaturated fatty acids oleic acid (18:1n − 9) and palmitoleic acid (16:1n − 7) were slightly higher in the control, whereas polyunsaturated fatty acids, except 22:6n − 3 and 20:5n − 3, were significantly greater in the transgenic fish (P< 0.05), similar to the results from the liver. However, by contrast to the liver, the major saturated fatty acids palmitic acid (16:0) and stearic acid (18:0) and polyunsaturated fatty acids (22:6n − 3) and (20:5n − 3) were significantly higher (P< 0.05) in the transgenic fish than in the controls.