5. Species-Specific Mechanisms of Neuron Subtype Specification Reveal Evolutionary Plasticity of Amniote Brain Development.
5. Species-Specific Mechanisms of Neuron Subtype Specification Reveal Evolutionary Plasticity of Amniote Brain Development.
复制标题
5.神经元亚型规范的物种特异性机制揭示了羊膜动物大脑发育的进化可塑性。
DOI：
10.1016/j.celrep.2018.02.086
复制
发表时间：
2018
期刊：
影响因子：
8.8
通讯作者：
Ono K.
中科院分区：
文献类型：
--
作者：
Nomura T;Yamashita W;Gotoh H;Ono K.
脊椎动物高度有序的脑结构由具有特定神经元连接的多个神经元亚型组成。然而，神经元特化机制的起源和进化变化仍不清楚。在这里，我们报告说，神经元亚型规范的监管机制是不同的发展中的神经元大脑。在哺乳动物新皮层中，转录因子（TF）Ctip2和Satb2在层特异性神经元中差异表达。相反，这些TF共同定位于爬行动物和鸟类背侧套膜神经元。产生不同神经元亚型的多潜能祖细胞通常存在于爬行动物和鸟类的背腭中，而avianCtip2的acis调节元件表现出减弱的转录抑制活性。此外，由这些TF区分的神经元亚型与神经元之间的保守神经元连接并不紧密相关。我们的研究结果揭示了调节基因功能的进化可塑性，这些功能导致了发育中的神经元异质性和连接性的物种差异。
Highly ordered brain architectures in vertebrates consist of multiple neuron subtypes with specific neuronal connections. However, the origin of and evolutionary changes in neuron specification mechanisms remain unclear. Here, we report that regulatory mechanisms of neuron subtype specification are divergent in developing amniote brains. In the mammalian neocortex, the transcription factors (TFs) Ctip2 and Satb2 are differentially expressed in layer-specific neurons. In contrast, these TFs are co-localized in reptilian and avian dorsal pallial neurons. Multi-potential progenitors that produce distinct neuronal subtypes commonly exist in the reptilian and avian dorsal pallium, whereas acis-regulatory element of avianCtip2exhibits attenuated transcription suppressive activity. Furthermore, the neuronal subtypes distinguished by these TFs are not tightly associated with conserved neuronal connections among amniotes. Our findings reveal the evolutionary plasticity of regulatory gene functions that contribute to species differences in neuronal heterogeneity and connectivity in developing amniote brains.