Microfluidic channels with renewable and switchable biological functionalities based on host-guest interactions.
Microfluidic channels with renewable and switchable biological functionalities based on host-guest interactions.
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DOI：
10.1039/c8tb02148a
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发表时间：
2018-12
期刊：
影响因子：
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通讯作者：
Siyuan Li;Bing Liu;Ting Wei;Changmin Hu;Yingjie Hang;Yishi Dong;Xiaoli Liu;Hong Chen
中科院分区：
文献类型：
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作者：
Siyuan Li;Bing Liu;Ting Wei;Changmin Hu;Yingjie Hang;Yishi Dong;Xiaoli Liu;Hong Chen
基于聚二甲基硅氧烷（PDMS）的微流体系统由于其易于制造、光学透明性和机械性能而受到越来越多的关注。然而，PDMS固有的疏水性和化学惰性阻碍了其在微流体系统中的更广泛应用。因此，迫切需要对基于 PDMS 的微流体通道进行表面修饰的方法。在这项工作中，使用简单的光化学过程，将低聚（乙二醇）甲基丙烯酸酯（OEGMA）和含金刚烷的OEGMA（OEGMA-Ada）接枝共聚在PDMS微通道表面上，得到PDMS-POA。选择 OEGMA 是因为它能抵抗非特异性蛋白质吸附，选择 OEGMA-Ada 是因为它随后能够附着具有细菌结合亲和力的甘露糖或具有亲和素结合亲和力的生物素。用生物素 (CD-B) 和/或甘露糖 (CD-M) 装饰的 β-CD 通过金刚烷和 β-CD 部分之间的主客体相互作用附着到 PDMS-POA 微通道上。获得的数据表明PDMS-POA/CD-B和PDMS-POA/CD-M微通道在生物素结合和细菌粘附方面的功能是可更新的。此外，PDMS-POA微通道的生物功能可以通过用SDS处理来转换以释放CD成分，然后用不同的β-CD衍生物处理。与之前基于PDMS的微流体通道的表面修饰策略不同，可见光诱导的接枝和主客体化学的结合为修饰的PDMS微通道提供了可再生和可切换的生物功能，用于检测和测量特定的蛋白质和细菌。
Poly(dimethylsiloxane) (PDMS)-based microfluidic systems are gaining increasing attention due to their ease of fabrication, optical transparency and mechanical properties. However, the inherent hydrophobicity and chemical inertness of PDMS hinder its wider application in microfluidic systems. There is thus a strong need for methods for surface modification of PDMS-based microfluidic channels. In this work, oligo(ethylene glycol)methacrylate (OEGMA) and adamantane-containing OEGMA (OEGMA-Ada) were graft copolymerized on PDMS microchannel surfaces using a simple photochemical process to give PDMS-POA. OEGMA was chosen for its resistance to non-specific protein adsorption, and OEGMA-Ada was chosen for its subsequent attachment of mannose with bacteria binding affinity or biotin with avidin binding affinity. β-CD decorated with biotin (CD-B) and/or mannose (CD-M) was attached to the PDMS-POA microchannels via host-guest interactions between the adamantane and β-CD moieties. The data obtained suggest that the functions of the PDMS-POA/CD-B and PDMS-POA/CD-M microchannels with respect to biotin binding and bacterial adhesion were renewable. In addition, the biofunction of the PDMS-POA microchannels could be switched by treatment with SDS to release the CD component followed by treatment with a different β-CD derivative. Different from previous surface modification strategies for PDMS-based microfluidic channels, the combination of visible light-induced grafting and host-guest chemistry provides modified PDMS microchannels with renewable and switchable biofunctions for the detection and measurement of specific proteins and bacteria.