Quantitation of trans fatty acids in milk fat using spectroscopic and chromatographic methods
Quantitation of trans fatty acids in milk fat using spectroscopic and chromatographic methods
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使用光谱和色谱方法定量乳脂中的反式脂肪酸
DOI：
10.1017/s0022029900028442
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发表时间：
1994
影响因子：
2.1
通讯作者：
M. Henninger
中科院分区：
文献类型：
--
作者：
F. Ulberth;M. Henninger
通过傅立叶变换i.r.（FTIR）光谱。当样品作为衍生的甲酯进行分析时，光谱减法技术导致TFA值不受完整三酰甘油的非特异性吸收或反式带形状的影响。奥地利乳脂样品含有20-50 g TFA/kg。通过分析TFA作为三酰基甘油获得的结果与通过使用甲酯获得的结果相比是有利的。FTIR方法的研究结果进一步证实了银化色谱法结合毛细管气相色谱法对同一组样品。除反式单烯酸外，非亚甲基中断的顺式、反式十八碳二烯酸对总反式含量贡献显着。通过银染色谱、气相色谱和氧化降解法研究了乳脂中反式-11，顺式-15十八碳二烯酸和顺式-9，反式-13十八碳二烯酸的保留行为。
Summary Trans fatty acids (TFA) formed during biohydrogenation by ruminant animals were quantified in cows' milk fat by means of Fourier transform i.r. (FTIR) spectroscopy. When samples were analysed as the derived methyl esters, a spectral subtraction technique resulted in TFA values that were not biased by the unspecific absorption of intact triacylglycerols or influenced by the shape of the trans band. Austrian milk fat samples contained 20–50 g TFA/kg. Results obtained by analysis of TFA as triacylglycerols compared favourably with those obtained by using methyl esters. The findings of the FTIR method were further substantiated by applying argentation chromatography in combination with capillary gas chromatography to the same set of samples. Besides trans monoenoic acids, non-methylene interrupted cis, trans octadecadienoic acids contributed significantly to the total trans content. Two novel TFA, trans-11, cis-15 octadecadienoic and cis-9, trans-13 octadecadienoic acids, were identified in milk fat by means of their retention behaviour on argentation chromatography and gas chromatography as well as by an oxidative degradation procedure.