TaARPC5 is required for wheat defense signaling in response to infection by the stripe rust fungus
TaARPC5 is required for wheat defense signaling in response to infection by the stripe rust fungus
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DOI：
10.1016/j.cj.2021.01.009
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发表时间：
2021-04
期刊：
影响因子：
6.6
通讯作者：
Jia Guo;Huan Peng;Tuo Qi;Sanding Xu;Md Ashraful Islam;B. Day;Qing Ma;Z. Kang;Jun Guo
中科院分区：
文献类型：
--
作者：
Jia Guo;Huan Peng;Tuo Qi;Sanding Xu;Md Ashraful Islam;B. Day;Qing Ma;Z. Kang;Jun Guo
结合共聚焦显微镜和药理学方法的大量研究表明，肌动蛋白细胞骨架对病原体感染做出动态反应。在这里，我们观察到鬼笔环肽处理诱导肌动蛋白成核，从而增强小麦对条锈病病原体条锈病的抗性。小麦属(Pst)。为了定义支撑这一过程的机制，我们表征了一个保守的肌动蛋白结合蛋白家族，即控制肌动蛋白聚合的肌动蛋白相关蛋白（ARP）家族。具体来说，我们鉴定并表征了小麦ARPC基因（TaARPC5），该基因编码含有P16-Arc结构域（ARP2/3复合物的最小亚基）的136个氨基酸的蛋白质。在用无毒Pst菌株感染植物以及用鞭毛蛋白（例如flg22）诱导后，诱导TaARPC5mRNA积累。亚细胞定位分析表明，TaARPC5 主要定位于皮质肌动蛋白细胞骨架，其精确的细胞定位表明其接近与肌动蛋白-细胞器界面相关的过程。在用有毒的 Pst 处理后，TaARPC5 敲除植物表现出 PTI 特异性 mRNA 表达的显着降低。相反，我们观察到在感染不相容的 Pstisolate 后，活性氧 (ROS) 积累的诱导增强，TaCAT1 表达减少。与酵母互补测定一起，当前的研究证明了 TaARPC5 通过调节宿主肌动蛋白细胞骨架，在小麦抗 Pstin 感染的抗性信号中发挥作用。
Numerous studies using a combination of confocal microscopic- and pharmacological-based approaches have demonstrated that the actin cytoskeleton dynamically responds to pathogen infection. Here, we observed that phalloidin treatment induced actin nucleation, resulting in enhanced resistance of wheat against the stripe rust pathogenPuccinia striiformisf. sp.tritici(Pst). To define the mechanism underpinning this process, we characterized a family of conserved actin-binding proteins, the actin related protein (ARP) family, which controls actin polymerization. Specifically, we identified and characterized a wheat ARPC gene (TaARPC5), which encodes a 136-amino acid protein containing a P16-Arc domain, the smallest subunit of the ARP2/3 complex.TaARPC5mRNA accumulation was induced following the infection of plants with the avirulentPststrain, and following the elicitation with flagellin (e.g., flg22) as well. Subcellular localization analysis revealed that TaARPC5 is primarily localized to the cortical actin cytoskeleton, and its precise cellular localizations suggest the proximity to processes correlated with the actin-organelle interface. Upon treatment with virulentPst,TaARPC5-knockdown plants exhibited a significant reduction in the expression of PTI-specific mRNAs. Conversely, we observed enhanced induction of reactive oxygen species (ROS) accumulation and a decrease inTaCAT1expression following infection with an incompatiblePstisolate. Together with yeast complementation assays, the current study demonstrates a role forTaARPC5in resistance signaling in wheat againstPstinfection by regulating the host actin cytoskeleton.