Identification of histone H2B as a potential receptor for Mycoplasma genitalium protein of adhesion
Identification of histone H2B as a potential receptor for Mycoplasma genitalium protein of adhesion
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鉴定组蛋白 H2B 作为生殖支原体粘附蛋白的潜在受体
DOI：
10.1093/femspd/ftab053
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发表时间：
2021
影响因子：
3.3
通讯作者：
Zeng Y
中科院分区：
文献类型：
--
作者：
Liao Y;Deng X;Peng K;Dai P;Luo D;Liu P;Chen L;Li X;Ye Y;Zeng Y
ABSTRACT. Mycoplasma genitalium, the smallest prokaryotic microorganism capable of independent replication, is increasingly recognized as a sexually transmitted pathogen. M. genitalium protein of adhesion (MgPa) plays a pivotal role in the process of M. genitalium adhesion to host cells. We previously identified cyclophilin A as a cellular receptor of MgPa using the virus overlay protein binding assay (VOPBA) together with liquid chromatography-mass spectrometry (LC-MS). In the current study, we have evaluated H2B as an alternative cellular receptor for MgPa since H2B was assigned the second higher score as a potential binding partner of MgPa in the VOPBA and LC-MS screen. It was found that recombinant MgPa specifically bind to H2B both in the SV-HUC-1 cell membrane and in form of a recombinant protein. H2B was detected throughout the SV-HUC-1 cells, including the cytoplasmic membrane, cytosol and nucleus. Importantly, H2B partially inhibited the adhesion of M. genitalium to SV-HUC-1 cells. Finally, H2B was both co-precipitated with recombinant MgPa and co-localized with M. genitalium and recombinant MgPa in SV-HUC-1 cells. The above observations suggest that H2B may act as a potential cellular receptor of MgPa for mediating M. genitalium adhesion to host cells.
