African swine fever: Morphopathology of a viral haemorrhagic disease
African swine fever: Morphopathology of a viral haemorrhagic disease
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DOI：
10.1136/vr.139.11.249
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发表时间：
1996-09-14
影响因子：
2.2
通讯作者：
Jover, A
中科院分区：
文献类型：
--
作者：
Rodriguez, F;Fernandez, A;Jover, A
用中等毒力的非洲猪瘟病毒E75西班牙分离株对12头小型猪进行实验感染。该疾病急性和亚急性复制，感染后6天，动物表现出严重的临床和病理学体征，特征为淋巴和非淋巴器官弥漫性出血。用两种不同的方法进行免疫组织病理学研究，并用单克隆抗体（18BG 3）分析病毒蛋白73（VP73）和IgM、IgG的分布。VP73主要在单核吞噬细胞系统的细胞中观察到，并且在许多巨核细胞、肾脏的一些肾小管上皮细胞、扁桃体上皮细胞、一些肝细胞中以及在感染后期的一些内皮细胞和中性粒细胞中观察到。组织学检查结果和分布的VP73和IgM和IgG表明，病毒引起出血性病变，通过其对各种止血机制的影响。
Twelve miniature pigs were infected experimentally with the moderately-virulent Spanish isolate of African swine fever virus E75. The disease was reproduced acutely and subacutely, and six days after infection the animals showed severe clinical and pathological signs characterised by diffuse haemorrhages in lymphoid and non-lymphoid organs. An immunohistopathological study, using two different methods, and a monoclonal antibody (mAb) (18BG3) were used to analyse the distribution of the virus protein 73 (VP73) and IgM and IgG. VP73 was observed primarily in cells of the mononuclear phagocyte system, and in many megakaryocytes, some tubular epithelial cells of the kidneys, tonsillar epithelium, some hepatocytes, and in a few endothelial cells and neutrophils in the later stages of the infection. The histological findings and the distribution of VP73 and IgM and IgG indicated that the virus caused haemorrhagic lesions through its effect on various haemostatic mechanisms.