Collagen-derived dipeptide prolyl-hydroxyproline promotes differentiation of MC3T3-E1 osteoblastic cells
Collagen-derived dipeptide prolyl-hydroxyproline promotes differentiation of MC3T3-E1 osteoblastic cells
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DOI：
10.1016/j.bbrc.2014.09.121
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发表时间：
2014-10-24
影响因子：
3.1
通讯作者：
Mano, Hiroshi
中科院分区：
文献类型：
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作者：
Kimira, Yoshifumi;Ogura, Kana;Mano, Hiroshi
羟脯氨酸(Pro-Hyp)是胶原源性二肽的主要成分之一。本研究旨在探讨羟丙基转移酶原对MC3T3-E1成骨细胞增殖和分化的影响。加入Pro-Hyp不影响MC3T3-E1细胞的增殖和基质矿化，但显着提高碱性磷酸酶的活性。此外，Pro-Hyp处理的细胞显著上调了Runx2 Osterix和col1α1的基因表达。这些结果表明Pro-Hyp促进成骨细胞的分化。这项研究首次证明了Pro-Hyp通过上调Runx2、Osterix和col1α1基因的表达而对成骨细胞的分化起到积极的作用。(C)2014 Elsevier Inc.保留所有权利。
Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides. The objective of this study was to investigate the effects of Pro-Hyp on the proliferation and differentiation of MC3T3-E1 osteoblastic cells. Addition of Pro-Hyp did not affect MC3T3-E1 cell proliferation and matrix mineralization but alkaline phosphatase activity was significantly increased. Furthermore, cells treated with Pro-Hyp significantly upregulated gene expression of Runx2 Osterix, and Col1 alpha 1. These results indicate that Pro-Hyp promotes osteoblast differentiation. This study demonstrates for the first time that Pro-Hyp has a positive effect on osteoblast differentiation with upregulation of Runx2, Osterix, and Col1 alpha 1 gene expression. (C) 2014 Elsevier Inc. All rights reserved.