Antibody to liver cytosol (anti‐LC1) in patients with autoimmune chronic active hepatitis type 2
Antibody to liver cytosol (anti‐LC1) in patients with autoimmune chronic active hepatitis type 2
复制标题
自身免疫性慢性活动性肝炎 2 型患者的肝细胞质抗体（抗 LC1）
作者：
É. Martini;N. Abuaf;Florence Cavalli;V. Durand;C. Johanet;J. Homberg
用免疫沉淀法检测21例慢性活动性肝炎患者血清中一种新的自身抗体。抗体在肝脏中与可溶性细胞质抗原发生反应。该抗体具有器官特异性而非物种特异性，因此被称为抗肝细胞质抗体1型（anti - LC1）。21例中有7例未发现其他自身抗体；其余14例有抗肝/肾微粒体抗体1型（抗LKM1）。采用间接免疫荧光法，7种血清中含有抗LC1和不含抗LKM1时，观察到不同的染色模式。该抗体对四种不同动物的肝细胞细胞质进行染色，并保留小鼠和大鼠肝脏中心静脉周围的细胞层，我们称之为近静脉肝细胞。肝细胞质组分吸收血清后，免疫荧光模式消失。含有这两种抗体的14份血清在肝微体部分吸收抗LKM1后显示出抗LC1的免疫荧光模式。抗LC1仅在原因不明的慢性活动性肝炎患者的血清中发现。100例慢性活动性肝炎合并抗肌动蛋白抗体的典型慢性活动性肝炎患者、1100例原发性胆汁性肝硬化患者、157例药物性肝炎患者以及大量肝脏和非肝脏疾病患者的血清中均未发现抗LC1抗体。这种新抗体被认为是与抗LKM1（抗LKM1慢性活动性肝炎）或自身免疫性慢性活动性肝炎2型相关的慢性活动性肝炎的第二标志物。
A new autoantibody was detected by immunoprecipitation in the serum of 21 patients with chronic active hepatitis. The antibody reacted against a soluble cytosolic antigen in liver. The antibody was organ specific but not species specific and was therefore called antiliver cytosol antibody Type 1 (anti‐LC1). In seven of 21 cases, no other autoantibody was found; the remaining 14 cases had anti‐liver/kidney microsome antibody Type 1 (anti‐LKM1). With indirect immunofluorescence, a distinctive staining pattern was observed with the seven sera with anti‐LC1 and without anti‐LKM1. The antibody stained the cytoplasm of hepatocytes from four different animal species and spared the cellular layer around the central veins of mouse and rat liver that we have called juxtavenous hepatocytes. The immunofluorescence pattern disappeared after absorption of sera by a liver cytosol fraction. The 14 sera with both antibodies displayed anti‐LC1 immunofluorescent pattern after absorption of anti‐LKM1 by the liver micro‐somal fraction. The anti‐LC1 was found in the serum only in patients with chronic active hepatitis of unknown cause. Anti‐LC1 antibody was not found in sera from 100 patients with chronic active hepatitis associated with anti‐actin antibody classic chronic active hepatitis Type 1, 100 patients with primary biliary cirrhosis, 157 patients with drug‐induced hepatitis and a large number of patients with liver and nonliver diseases. This new antibody was considered a second marker of chronic active hepatitis associated with anti‐LKM1 (anti‐LKM1 chronic active hepatitis) or autoimmune chronic active hepatitis Type 2.