Mosquito age and susceptibility to insecticides
Mosquito age and susceptibility to insecticides
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DOI：
10.1016/j.trstmh.2011.01.009
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发表时间：
2011-05-01
影响因子：
2.2
通讯作者：
Ranson, Hilary
中科院分区：
文献类型：
--
作者：
Rajatileka, Shavanthi;Burhani, Joseph;Ranson, Hilary
杀虫剂在控制蚊媒疾病的传播方面发挥着至关重要的作用，杀虫剂抗药性的发展和传播是可持续控制的主要威胁。世界卫生组织制定的监测杀虫剂耐药性的指南建议使用 1-3 天大的非吸血雌性蚊子。这种标准化有助于不同测试之间的比较，这在监测现场电阻的空间或纵向变化时非常重要。然而，这个年龄的蚊子不能传播人类病原体。为了传播疾病，蚊子必须活得足够长，才能通过吸血吸收病原体，度过外部潜伏期，然后在随后的吸血过程中传播病原体。此前的研究报告称，随着蚊子年龄的增长，杀虫剂耐药性会下降。如果广泛适用，这将对基于世界卫生组织生物测定结果的耐药性影响的预测产生重要影响。这项研究调查了衰老和吸血对六个不同蚊子种群中杀虫剂引起的死亡率的影响，发现三个埃及伊蚊种群和两个冈比亚按蚊种群的老年蚊子在接触杀虫剂后死亡率更高。研究人员对已知杀虫剂解毒基因 GSTe2 的表达以及靶位点突变 (kdr 1014F) 频率的年龄依赖性变化进行了研究，试图解释结果。 (C) 2011 年皇家热带医学和卫生学会。由爱思唯尔有限公司出版。保留所有权利。
Insecticides play a crucial role in controlling the transmission of mosquito-borne diseases and the development and spread of insecticide resistance is a major threat to sustainable control. Guidelines developed by the WHO to monitor for insecticide resistance recommend using 1-3 day old, non blood fed female mosquitoes. This standardisation facilitates comparison between different tests, which is important when monitoring for spatial or longitudinal variations in resistance in the field. However, mosquitoes of this age cannot transmit human pathogens. In order to transmit disease, the mosquito must live long enough to pick up the pathogen via a blood meal, survive the extrinsic incubation period and then pass on the pathogen during a subsequent blood meal. Previous studies have reported declines in insecticide resistance with mosquito age. If widely applicable this would have important implications for predictions of the impact of resistance that are based on results from WHO bioassays. This study investigated the impact of senescence and blood feeding on insecticide induced mortality in six different mosquito populations and found higher mortality after insecticide exposure in older mosquitoes in three populations of Aedes aegypti and two Anopheles gambiae populations. Age dependent changes in the expression of a known insecticide detoxification gene, GSTe2, and in the frequency of a target site mutation (kdr 1014F) were investigated in an attempt to explain the results. (C) 2011 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.