ENDOTHELIN IS A POTENT SECRETAGOGUE FOR ATRIAL NATRIURETIC PEPTIDE IN CULTURED RAT ATRIAL MYOCYTES
ENDOTHELIN IS A POTENT SECRETAGOGUE FOR ATRIAL NATRIURETIC PEPTIDE IN CULTURED RAT ATRIAL MYOCYTES
复制标题
DOI：
10.1016/s0006-291x(88)81064-7
复制
发表时间：
1988-08-30
影响因子：
3.1
通讯作者：
MASAKI, T
中科院分区：
文献类型：
--
作者：
FUKUDA, Y;HIRATA, Y;MASAKI, T
使用培养的新生大鼠心房心细胞，我们研究了合成猪内皮素（PET）的作用，这是一种从内皮细胞中分离出的新型有效血管收缩剂，对释放免疫反应性（IR）大鼠心房natriaretic肽（RANP）的释放。 PET以剂量依赖性的方式（10-10-10-7 m）刺激IR-RANP分泌，大约半最大的刺激性剂量为2时。 10-10 M. PET诱导的IR-RANP分泌被Ca2+ - 通道阻滞剂尼卡迪平衰减，但与Ca2+ - 通道激动剂Bay-K 8644结合使用时，没有进一步的刺激。导致对IR-RANP分泌产生协同作用。这些数据表明，ET可以通过在心房心脏细胞中的电压依赖性Ca2+通道来调节Ca2+涌入来作为RANP的内源性分泌。
Using cultured neonatal rat atrial cardiocytes, we have studied the effect of synthetic porcine endothelin (pET), a novel potent vasoconstrictor isolated from endothelial cells, on the release of immunoreactive (IR) rat atrial natriuretic peptide (rANP). pET stimulated IR-rANP secretion in a dose-dependent manner (10-10-10-7 M) with an approximate half-maximally stimulatory dose of 2 .times. 10-10 M. The pET-induced IR-rANP secretion was attenuated by Ca2+-channel blocker nicardipine, but no further stimulation was induced when combined with a Ca2+-channel agonist BAY-K 8644. pET in combination with tetradecanoyl-phorbol-acetate resulted in a synergistic effect on IR-rANP secretion. These data suggest that ET may play as an endogenous secretagogue for rANP by modulating Ca2+ influx through the voltage-dependent Ca2+-channels in atrial cardiocytes.