Human regional lymph nodes draining cancer exhibit a profound dendritic cell depletion as comparing to those from patients without malignancies
Human regional lymph nodes draining cancer exhibit a profound dendritic cell depletion as comparing to those from patients without malignancies
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DOI：
10.1016/s0165-2478(02)00172-4
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发表时间：
2002-12-03
影响因子：
4.4
通讯作者：
Di Girolamo, V
中科院分区：
文献类型：
--
作者：
Laguens, G;Coronato, S;Di Girolamo, V
树突状细胞（Dendritic cells，DC）是骨髓来源的专职抗原提呈细胞（antigen presenting cells，APC）。它们被认为是能够诱导初次免疫应答的最有效的APC。DC有效地捕获和处理蛋白质和非蛋白质抗原。它们广泛分布于全身并占据前哨位置，如上皮细胞。针对癌症的免疫应答的建立可能取决于DC将肿瘤抗原转移（捕获、加工和呈递）到区域淋巴结中的能力，在区域淋巴结中它们可以诱导导致肿瘤排斥的特异性应答。因为宿主“专业”DC是诱导特异性抗肿瘤应答的最重要因素之一，而淋巴结是发生免疫应答的地方，我们采用免疫组织化学方法，用抗-CD 44单克隆抗体检测了47例不同恶性上皮性肿瘤患者的无转移淋巴结和11例非恶性肿瘤患者的淋巴结中DCs的密度。S100蛋白、CD 86和CD 1a抗体。通过形态计量学分析，我们观察到肿瘤患者区域淋巴结中S100+和CD 1a + DCs密度与对照淋巴结相比显著降低（P分别< 0.0001和0.003）。引流癌淋巴结中S100+ DC和CD 86 + DC密度相似。总之，这些数据表明，引流癌的淋巴结具有比S100+和可能的CD 86 + DC显著更少的CD 1a + DC。这些发现可能代表了肿瘤逃避免疫识别的另一种机制。(C)2002 Elsevier Science B. V.保留所有权利。
Dendritic cells (DCs) are bone-marrow derived 'professional' antigen presenting cells (APC). They are considered as the most potent APC able to induce primary immune responses. DC efficiently capture and process proteic and non-proteic antigens. They are widely distributed throughout the body and occupy sentinel positions such as epithelia. Establishment of an immune response against cancer may depend of the capacity of DCs to transfer (to capture, to process and to present) tumor antigens into regional lymph nodes where they can induce a specific response leading to tumor rejection. Because host 'professional' DCs are one of the most important elements in the induction of specific anti-tumor responses and lymph nodes are the places where the immune response takes place, we investigated the densities of DCs within regional metastasis-free lymph nodes from 47 patients with different malignant epithelial tumors as comparing with lymph nodes from 11 patients without malignancies using an immunohistochemistry method with anti-S100 protein, CD86 and CD1a antibodies. By means of morphometric analysis, we observed that S100+ and CD1a+ DCs densities in regional lymph nodes from cancer patients were significatively decreased as compared with control lymph nodes (P < 0.0001 and 0.003, respectively). S100+ DCs and CD86+ DCs densities in lymph nodes draining cancer were similar. Taken together, these data indicated that lymph nodes draining cancer had significantly less CD1a+ DCs than S100+ and possibly CD86+ DCs. These findings may represent another mechanism by which tumors evade the immune recognition. (C) 2002 Elsevier Science B.V. All rights reserved.