LOCALIZATION AND CHARACTERIZATION OF PANCREATIC-POLYPEPTIDE RECEPTORS IN RAT ADRENAL-GLANDS
LOCALIZATION AND CHARACTERIZATION OF PANCREATIC-POLYPEPTIDE RECEPTORS IN RAT ADRENAL-GLANDS
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DOI：
10.1152/ajpgi.1992.262.3.g532
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发表时间：
1992-03-01
影响因子：
--
通讯作者：
TAYLOR, IL
中科院分区：
文献类型：
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作者：
WHITCOMB, DC;VIGNA, SR;TAYLOR, IL
胰多肽（PP）是在餐后或应激时从胰岛中释放出来的。 虽然PP表现出广泛的生物学效应，但很少（如果有的话）是由PP对其最终靶器官的直接作用介导的。 最近，PP受体已被确定在具有不完全血脑屏障的脑区域中，这表明PP可能通过中枢神经系统间接起作用。 在本研究中，我们试图通过体内放射受体测定和体外放射自显影来确定外周PP结合位点。 使用这些技术，我们已经确定了饱和的结合位点PP在束状肌，网状肌，和髓质的大鼠肾上腺。 我们的特点是这些网站使用膜放射性配体结合的平衡分析和放射性配体结合冷冻组织切片的定量放射自显影。 PP与这些位点的结合是可饱和的，具有高亲和力和特异性以及时间、温度和膜依赖性。 此外，PP结合到这些假定的受体位点在体内的生理浓度。 事实证明，人民党的许多行动都是间接的。 由于PP受体已被确定在大脑中，现在在肾上腺中，我们认为，PP的一些影响可能是通过直接和间接调制的大脑和肾上腺轴介导的。
Pancreatic polypeptide (PP) is released from pancreatic islets after meals or in response to stress. Although PP exhibits a wide spectrum of biological effects, few, if any, are mediated by a direct action of PP on its ultimate target organ. Recently, PP receptors have been identified in areas of the brain with an incomplete blood-brain barrier suggesting that PP may act indirectly through the central nervous system. In the present study, we sought to identify peripheral PP binding sites using an in vivo radioreceptor assay and in vitro autoradiography. Using these techniques, we have identified saturable binding sites for PP in the zona fasciculata, zona reticularis, and the medulla of the rat adrenal gland. We have characterized these sites using equilibrium analysis of membrane-radioligand binding and by quantitative autoradiography of radioligand binding to frozen tissue sections. Binding of PP to these sites is saturable, of high affinity, and specific as well as time, temperature, and membrane dependent. Moreover, PP binds to these putative receptor sites in vivo at physiological concentrations. Many of the actions of PPs have been demonstrated to be indirect. Because PP receptors have been identified in the brain and now in the adrenal gland, we suggest that some of the effects of PP may be mediated through direct and indirect modulation of the brain and adrenal axis.