Lytic potential of Lysobacter capsici VKM B-2533T: bacteriolytic enzymes and outer membrane vesicles
Lytic potential of Lysobacter capsici VKM B-2533T: bacteriolytic enzymes and outer membrane vesicles
复制标题
辣椒溶杆菌VKM B-2533T的裂解能力：溶菌酶和外膜囊泡
DOI：
10.1038/s41598-020-67122-2
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发表时间：
2020-06-19
影响因子：
4.6
通讯作者：
Vasilyeva, N. V.
中科院分区：
文献类型：
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作者：
Afoshin, A. S.;Kudryakova, I., V;Vasilyeva, N. V.
最近反复爆发的细菌对抗生素的耐药性表明，迫切需要找到新的裂解剂来对抗它们。辣椒溶杆菌VKM B-2533(T)对多种细菌、真菌和酵母菌具有较强的抑菌作用。其活性可归因于溶菌酶、抗生素和多肽的影响。本工作分离了四种均一的溶菌酶和两种蛋白质的混合物，它们也具有溶菌活性。这些分离株包括与L酶基因相同的蛋白质、α和β裂解酶以及赖氨酸专一性的蛋白酶。溶杆菌中26 kDa和29 kDa的蛋白以及一种被鉴定为N-乙酰糖胺酶的蛋白此前未被分离到。分离的β-裂解酶高效消化耐甲氧西林葡萄球菌活细胞(最低抑菌浓度为2.85 mU g/m L)。这一特性使得这种酶值得特别关注。获得了一种重组β-裂解酶。细菌的抗菌能力是由外膜小泡(OMV)贡献的。辣椒乳杆菌细胞形成一群具有抗真菌活性的OMV。这些数据表明，这种细菌具有巨大的抗菌潜力，需要进行深入的研究。
Recent recurrent outbreaks of bacterial resistance to antibiotics have shown the critical need to identify new lytic agents to combat them. The species Lysobacter capsici VKM B-2533(T) possesses a potent antimicrobial action against a number of bacteria, fungi and yeasts. Its activity can be due to the impact of bacteriolytic enzymes, antibiotics and peptides. This work isolated four homogeneous bacteriolytic enzymes and a mixture of two proteins, which also had a bacteriolytic activity. The isolates included proteins identical to L. enzymogenes alpha- and beta -lytic proteases and lysine-specific protease. The proteases of 26kDa and 29kDa and a protein identified as N-acetylglycosaminidase had not been isolated in Lysobacter earlier. The isolated beta -lytic protease digested live methicillin-resistant staphylococcal cells with high efficiency (minimal inhibitory concentration, 2.85 mu g/mL). This property makes the enzyme deserving special attention. A recombinant beta -lytic protease was produced. The antimicrobial potential of the bacterium was contributed to by outer membrane vesicles (OMVs). L. capsici cells were found to form a group of OMVs responsible for antifungal activity. The data are indicative of a significant antimicrobial potential of this bacterium that requires thorough research.