The 20 bp, directly repeated DNA sequence of broad host range plasmid R1162 exerts incompatibility in vivo and inhibits R1162 DNA replication in vitro.
The 20 bp, directly repeated DNA sequence of broad host range plasmid R1162 exerts incompatibility in vivo and inhibits R1162 DNA replication in vitro.
复制标题
广泛宿主范围质粒 R1162 的 20 bp、直接重复 DNA 序列在体内发挥不相容性，并在体外抑制 R1162 DNA 复制。
DOI：
10.1007/bf00328129
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Meyer,RJ
中科院分区：
文献类型：
--
作者：
Lin,LS;Kim,YJ;Meyer,RJ
宽宿主范围质粒R1162在质粒复制和维持所需的切孔区域含有直接重复的20bp DNA序列。该序列经化学合成和克隆，足以表达质粒不亲和性。该序列还抑制R1162 DNA在无细胞系统中的复制。这两种效应的强度取决于直接重复序列（dr）的数量，并且也受到重复序列中不同突变的相似程度的影响。在细胞中测试了几个突变对切切质粒维持的影响，发现一个突变导致质粒拷贝数增加。结果表明，直接重复序列通过抑制DNA复制而产生不相容性，可能是因为它们是限制性必需蛋白的结合位点。
The broad host range plasmid R1162 contains a directly repeated, 20 bp DNA sequence in the region of the plasmid required incisfor replication and maintenance. This sequence has been chemically synthesized and cloned, and shown to be sufficient for expression of plasmid incompatibility. The sequence also inhibits replication of R1162 DNA in a cell-free system. The strengths of both these effects are determined by the number of direct repeats (DRs) present, and are also affected to similar degrees by different mutations within the repeated sequence. Several of the mutations were tested for their effect incison plasmid maintenance in the cell, and one was found to cause an increase in plasmid copy number. The results suggest that the direct repeats exert incompatibility by inhibiting DNA replication, presumably because they are the binding sites for a limiting essential protein.