Protective Effect of Apple Polyphenols against Stress-Provoked Influenza Viral Infection in Restraint Mice
Protective Effect of Apple Polyphenols against Stress-Provoked Influenza Viral Infection in Restraint Mice
复制标题
苹果多酚对应激小鼠流感病毒感染的保护作用
DOI：
10.1021/jf104982y
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发表时间：
2011-04-27
影响因子：
6.1
通讯作者：
Kurihara, Hiroshi
中科院分区：
文献类型：
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作者：
He, Rong-Rong;Wang, Min;Kurihara, Hiroshi
本研究旨在探讨苹果多酚提取物（APE）对抑制应激小鼠的抗流感病毒作用。对其进行高效液相色谱指纹图谱分析，测定其多酚类成分占比为81.796。我们的研究结果表明，约束应激显著提高了感染H1N1病毒的小鼠的死亡率和并发症的持续时间。然而，口服APE（100和200 mg/kg）可提高流感病毒感染应激小鼠的存活率和延长生存时间，且呈剂量依赖性。进一步发现，APE能显著提高抑制应激小鼠脾脏免疫细胞数量、CD4辅助细胞比例、IL-2分泌和自然杀伤（NK）细胞毒性（LU10/脾）能力。此外，APE还显著降低了脾细胞脂质过氧化水平，提高了氧自由基吸收能力（ORAC）。这些结果表明，APE对流感病毒感染小鼠的保护作用可能与减轻应激性免疫功能损伤有关，其抗氧化作用可能有助于免疫功能的恢复。
This study was conducted to investigate the effects of apple polyphenol extract (APE) against influenza virus in mice loaded with restraint stress. The high-performance liquid chromatography (HPLC) fingerprint of APE was recorded, and the percentage composition of polyphenols was determined as 81.796. Our results showed that restraint stress significantly promoted the mortality and duration of complications of mice infected with the H1N1 virus. However, oral administration of APE (100 and 200 mg/kg) improved the survival rates and prolonged living time of stressed mice infected with influenza virus in a dose-dependent manner. APE was further found to significantly improve the number of immunocytes, ratio of CD4 helper cells, secretion of IL-2, and capabilities of natural killer (NK) cytotoxicity (LU10/spleen) in spleens of restraint-stressed mice. In addition, APE also significantly decreased the level of lipid peroxidation and increased oxygen radical absorbance capacity (ORAC) in splenocytes. These results indicated that the protective effects of APE on mice infected with influenza virus were related to the alleviation of stress-induced impairment of immune functions and its antioxidant property might contribute to the immune recovery.