Identification of an S-antigen-like molecule in human choroid plexus and cerebrospinal fluid.
Identification of an S-antigen-like molecule in human choroid plexus and cerebrospinal fluid.
复制标题
人脉络丛和脑脊液中 S 抗原样分子的鉴定。
DOI：
10.1038/eye.1992.128
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Donoso,LA
中科院分区：
文献类型：
--
作者：
Dua,HS;Reyes,PF;Barrett,JA;Abrams,MS;Schwarting,R;Craft,CM;Donoso,LA
对视网膜S抗原的敏感性与几种临床形式的后葡萄膜炎的发病机制有关。最近在实验动物的脑和脉络丛中发现了S抗原样分子。为了鉴定人脉络丛和脑脊液中的S抗原样分子，我们用4株单抗MAbF4-C1、MAbC10-C10、MAbA2-G5和MAbA9-C6，它们分别定义了S抗原的氨基、中位和羧基末端的特异性表位。三株单抗MAbF4-C1、MAbC10-C10和MAbA9-C6将S样抗原样分子定位于人脉络丛上皮细胞的细胞质。聚合酶链式反应证实脉络丛中存在S抗原同源物。用免疫印迹法检测了7例不同神经病变患者的脑脊液标本，结果表明脑脊液中存在S抗原样分子。作者认为，视网膜S抗原与人脉络丛和脑脊液中S抗原样分子之间的免疫交叉反应和生化相似性，可能是某些临床类型葡萄膜炎的神经学表现的基础。
Sensitisation to retinal S-antigen has been implicated in the pathogenesis of several clinical forms of posterior uveitis. S-antigen-like molecules have recently been demonstrated in the brain and choroid plexus of experimental animals. We used a panel of four monoclonal antibodies (MAbs), MAbF4-C1, MAbC10-C10, MAbA2-G5 and MAbA9-C6, which define specific epitopes in the amino, mid and carboxyl terminal portions of S-antigen in order to identify an S-antigen-like molecule in human choroid plexus and cerebrospinal fluid (CSF). Three MAbs, MAbF4-C1, MAbC10-C10 and MAbA9-C6, localised an S-antigen-like molecule to the cytoplasm of the epithelial cells of the human choroid plexus. Polymerase chain reaction of cDNA from choroid plexus verified the presence of S-antigen homologues in the choroid plexus. The presence of an S-antigen-like molecule in the CSF was demonstrated by western blots in seven CSF samples from patients with a variety of neuropathological disorders. It is proposed that immunological cross-reactivity and biochemical similarity between retinal S-antigen and an S-antigen-like molecule in human choroid plexus and CSF could form a basis for neurological manifestations observed in certain clinical forms of uveitides.