VACCINIA VIRUS INDUCES CELLULAR MESSENGER-RNA DEGRADATION
VACCINIA VIRUS INDUCES CELLULAR MESSENGER-RNA DEGRADATION
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DOI：
10.1128/jvi.47.3.529-539.1983
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
ROBERTS, BE
中科院分区：
文献类型：
--
作者：
RICE, AP;ROBERTS, BE
牛痘病毒对小鼠L细胞的感染诱导了细胞多肽合成的快速抑制和蛋白质合成转向病毒多肽的专属生产。这种细胞特异性蛋白质合成的关闭是通过病毒诱导细胞mRNA快速降解的新机制实现的。在细胞mRNA降解的同时，病毒继续有序地表达其自身的遗传信息。利用高度保守的c[互补]DNA序列编码鸡β，评估了牛痘病毒感染对2个丰富的l细胞mRNA的影响。-肌动蛋白和-微管蛋白。杂交分析表明，在整个感染过程中，这两种mRNA都有快速和渐进的降解。感染3小时后，它们在未感染L细胞中的浓度降低到< 50%，在8至10小时之间，它们几乎完全降解。这一观察结果部分解释了牛痘病毒抑制宿主细胞蛋白质合成的机制。
The infection of mouse L cells with vaccinia virus induced induced a rapid inhibition of cellular polypeptide synthesis and a diversion of protein synthesis to the exclusive production of viral polypeptides. This shutoff of cell-specific protein synthesis was achieved by a novel mechanism by which the virus induced the rapid degradation of cellular mRNA. Concurrent with the degradation of cellular mRNA, the virus proceeds in the orderly temporal expression of its own genetic information. The effect of vaccinia virus infection upon 2 abundant L-cell mRNA was assessed by using the highly conserved c[complementary]DNA sequences that encode chicken .beta.-actin and rat .alpha.-tubulin. Hybridization analyses demonstrated that throughout infection there is a rapid and progressive degradation of both of these mRNA. After 3 h of infection they are reduced to < 50% of their concentration in uninfected L cells, and between 8 to 10 h they are almost entirely degraded. This observation explains in part the mechanism by which vaccinia virus inhibits host cell protein synthesis.