Hydrogen inhalation therapy regulates lactic acid metabolism following subarachnoid hemorrhage through the HIF-1α pathway.
Hydrogen inhalation therapy regulates lactic acid metabolism following subarachnoid hemorrhage through the HIF-1α pathway.
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DOI：
10.1016/j.bbrc.2023.04.072
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发表时间：
2023-04
影响因子：
3.1
通讯作者：
Zheng Peng;Xiaojian Li;Cong Pang;Jia-Tong Zhang;Qigai Zhu;Jianling Sun;Juan Wang;Bo-qiang Cao;Yu-hua Zhang;Yue Lu;Wei Li;C. Hang;Zhuang Zong
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文献类型：
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作者：
Zheng Peng;Xiaojian Li;Cong Pang;Jia-Tong Zhang;Qigai Zhu;Jianling Sun;Juan Wang;Bo-qiang Cao;Yu-hua Zhang;Yue Lu;Wei Li;C. Hang;Zhuang Zong
氢的神经保护作用已被证实，但其机制仍知之甚少。在一项对蛛网膜下腔出血（SAH）患者吸入氢气的临床试验中，我们发现氢气可以减少神经系统中乳酸的积累。目前还没有研究证明氢对乳酸的调节作用，我们希望通过本研究进一步阐明氢调节乳酸代谢的机制。细胞实验中，PCR和Western Blot结果显示HIF-1α是氢干预前后乳酸代谢变化最大的靶标。氢干预治疗抑制HIF-1α水平。激活HIF-1α抑制氢的降乳酸作用。我们还在动物实验中证明了氢的降乳酸作用。我们的工作阐明了氢可以通过hif -1α途径调节乳酸代谢，为氢的神经保护机制提供了新的见解。
The neuroprotective effects of hydrogen have been demonstrated, but the mechanism is still poorly understood. In a clinical trial of inhaled hydrogen in patients with subarachnoid hemorrhage (SAH), we found that hydrogen reduced the accumulation of lactic acid in the nervous system. There are no studies demonstrating the regulatory effect of hydrogen on lactate and in this study we hope to further clarify the mechanism by which hydrogen regulates lactate metabolism. In cell experiments, PCR and Western Blot showed that HIF-1α was the target related to lactic acid metabolism that changed the most before and after hydrogen intervention. HIF-1α levels were suppressed by hydrogen intervention treatment. Activation of HIF-1α inhibited the lactic acid-lowering effect of hydrogen. We have also demonstrated the lactic acid-lowering effect of hydrogen in animal studies. Our work clarifies that hydrogen can regulate lactate metabolism via the HIF-1αpathway, providing new insights into the neuroprotective mechanisms of hydrogen.