Retrotransposons regulate host genes in mouse oocytes and preimplantation embryos
Retrotransposons regulate host genes in mouse oocytes and preimplantation embryos
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DOI：
10.1016/j.devcel.2004.09.004
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发表时间：
2004-10-01
影响因子：
11.8
通讯作者：
Knowles, BB
中科院分区：
文献类型：
--
作者：
Peaston, AE;Evsikov, AV;Knowles, BB
对哺乳动物成熟卵母细胞中转座因子（TE）表达的全面分析表明，LTR III类逆转录转座子对母体mRNA库做出了意想不到的高贡献，该库持续存在于卵裂期胚胎中。小鼠卵母细胞中最丰富的转录物来自小鼠转录物（MT）逆转录转座子家族，并且该家族和其他TE家族的表达受发育调节。此外，TE作为替代启动子和宿主基因的第一外显子，调节它们在完全生长的卵母细胞和卵裂期胚胎中的表达。据我们所知，这是第一个在哺乳动物中启动多个基因同步、发育调节表达的TE的例子。我们建议，差异TE表达触发顺序重编程的胚胎基因组在卵母细胞到胚胎的过渡和植入前胚胎。
A comprehensive analysis of transposable element (TE) expression in mammalian full-grown oocytes reveals that LTR class III retrotransposons make an unexpectedly high contribution to the maternal mRNA pool, which persists in cleavage stage embryos. The most abundant transcripts in the mouse oocyte are from the mouse transcript (MT) retrotransposon family, and expression of this and other TE families is developmentally regulated. Furthermore, TEs act as alternative promoters and first exons for a subset of host genes, regulating their expression in full-grown oocytes and cleavage stage embryos. To our knowledge, this is the first example of TEs initiating synchronous, developmentally regulated expression of multiple genes in mammals. We propose that differential TE expression triggers sequential reprogramming of the embryonic genome during the oocyte to embryo transition and in preimplantation embryos.