Determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy
Determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy
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DOI：
10.1016/s0308-8146(99)00191-0
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发表时间：
2000-02-01
期刊：
影响因子：
8.8
通讯作者：
Proctor, A
中科院分区：
文献类型：
--
作者：
Gnanasambandam, R;Proctor, A
建立了一种漫反射傅里叶变换红外光谱法（DRIFTS）测定市售果胶酯化度的方法。研究了红外光谱数据与滴定法测定的DE值之间的关系。酯羰基带面积（C=O）出现在1756 cm（-1）的平均频率与观察到的带的平均DE具有最高的相关性（R-2 = 0.822）。果胶样品的DE值从线性拟合方程计算的那些从滴定法得到的是可比的。DE 71.6、DE 62和DE 55.3的平均DE值分别在滴定法所得值的3.23%、2.9%和1%以内。在使用FTIR建立预测方程时，必须考虑样品来源引起的光谱变化。DRIFTS可作为果胶DE滴定分析的快速替代方法。(C)1999爱思唯尔科技有限公司。保留所有权利。
A diffuse reflectance Fourier transform infrared spectroscopy (DRIFTS) method was developed to measure the degree of esterification (DE) of commercial pectin samples. The relationship between infrared spectroscopy data and titrimetrically determined DE values was investigated. The ester carbonyl band area (C=O) occurring at a mean frequency of 1756 cm(-1) had the highest correlation (R-2 = 0.822) with the mean DE of the bands observed. The DE values of pectin samples calculated from the line fit equation were comparable to those obtained from the titrimetric method. Mean DE values obtained were within 3.23% for DE 71.6, 2.9% for DE 62, and less than 1% for DE 55.3 of values obtained by titrimetric method. Spectral variations due to sample source have to be considered in developing prediction equations using FTIR. DRIFTS can be a rapid, alternative method to titrimetric analysis of pectin DE. (C) 1999 Elsevier Science Ltd. All rights reserved.