High speed detection of circulating tumor cells
High speed detection of circulating tumor cells
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DOI：
10.1016/j.bios.2005.12.024
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发表时间：
2006-04-15
影响因子：
12.6
通讯作者：
Nieva, J
中科院分区：
文献类型：
--
作者：
Hsieh, HB;Marrinucci, D;Nieva, J
上皮肿瘤细胞在癌症患者的外周血中以超低浓度循环。我们开发了一种能够利用光纤阵列扫描技术 (FAST) 快速准确地检测循环中稀有细胞的仪器。 FAST 细胞仪可以在玻璃基板上定位免疫荧光标记的稀有细胞，扫描速度比传统自动数字显微镜快 500 倍。这些高扫描速率是通过使用具有大视场（50mm）的光纤束收集荧光发射来实现的。非常高的扫描速率使得无需富集步骤即可检测罕见事件的能力成为可能。 FAST 细胞仪用于对乳腺癌患者外周血中的循环肿瘤细胞进行检测、成像和重新成像。该技术有潜力成为临床上有用的即时诊断和癌症临床医生的预后工具。固定基质的使用有助于细胞的重新识别和重新染色，从而可以从先前收集和识别的细胞中获得额外的形态和生物学信息。 (c) 2006 Elsevier B.V. 保留所有权利。
Epithelial tumor cells circulate in peripheral blood at ultra-low concentrations in cancer patients. We have developed an instrument capable of rapid and accurate detection of rare cells in circulation utilizing fiber-optic array scanning technology (FAST). The FAST cytometer can locate immunofluorescently labeled rare cells on glass substrates at scan rates 500 times faster than conventional automated digital microscopy. These high scan rates are achieved by collecting fluorescent emissions using a fiber bundle with a large (50mm) field of view. Very high scan rates make possible the ability to detect rare events without the requirement for an enrichment step. The FAST cytometer was used to detect, image and re-image circulating tumor cells in peripheral blood of breast cancer patients. This technology has the potential to serve as a clinically useful point-of-care diagnostic and a prognostic tool for cancer clinicians. The use of a fixed substrate pert-nits the re-identification and re-staining of cells allowing for additional morphologic and biologic information to be obtained from previously collected and identified cells. (c) 2006 Elsevier B.V. All rights reserved.