An AP2/ERF gene, IbRAP2-12, from sweetpotato is involved in salt and drought tolerance in transgenic Arabidopsis
An AP2/ERF gene, IbRAP2-12, from sweetpotato is involved in salt and drought tolerance in transgenic Arabidopsis
复制标题
来自甘薯的 AP2/ERF 基因 IbRAP2-12 参与转基因拟南芥的耐盐和耐旱性
DOI：
10.1016/j.plantsci.2019.01.009
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发表时间：
2019-04-01
期刊：
影响因子：
5.2
通讯作者：
He, Shaozhen
中科院分区：
文献类型：
--
作者：
Li, Yan;Zhang, Huan;He, Shaozhen
APETALA 2/乙烯应答因子（AP 2/ERF）基因的操纵在植物抗非生物胁迫方面发挥了重要作用。本研究从耐盐甘薯品系ND 98中克隆了一个AP 2/ERF基因，命名为IbRAP 2 -12。在烟草表皮细胞中瞬时表达的IbRAP 2 -12蛋白在核中表达，在酵母中异源表达的IbRAP 2 -12蛋白具有转录激活作用。IbRAP 2 -12由NaCl（200 mM）、20%聚乙二醇（PEG）6000、100 μ M脱落酸（阿坝）、100 pM乙烯利和100 μ M茉莉酸甲酯（MeJA）诱导。IbRAP 2 -12过表达的拟南芥品系比野生型植物更耐受盐和干旱胁迫。转录组分析表明，盐胁迫和干旱胁迫下，IbRAP 2 -12过表达系中阿坝信号转导、JA信号转导、脯氨酸生物合成和活性氧（ROS）清除过程相关基因表达上调。在盐胁迫和干旱胁迫下，转基因株系的阿坝、JA和脯氨酸含量均显著增加，而过氧化氢（14202）含量和失水速率显著降低。这些结果表明IbRAP 2 -12在提高转基因拟南芥耐盐性和耐旱性中的作用。因此，该IbRAP 2 -12基因可用于提高植物在非生物胁迫期间的耐受能力。
The manipulation of APETALA2/ethylene responsive factor (AP2/ERF) genes in plants makes great contributions on resistance to abiotic stresses. Here, we cloned an AP2/ERF gene from the salt-tolerant sweetpotato line ND98 and named IbRAP2-12. IbRAP2-12 protein expressed in nuclear revealed by transient expression in tobacco epidermal cells, and IbRAP2-12 exhibited transcriptional activation using heterologous expression assays in yeast. IbRAP2-12 was induced by NaCl (200 mM), 20% polyethylene glycol (PEG) 6000, 100 mu M abscisic acid (ABA), 100 pM ethephon and 100 mu M methyl jasmonate (MeJA). IbRAP2-12-overexpressing Arabidopsis lines were more tolerant to salt and drought stresses than wild type plants. Transcriptome analysis showed that genes involved in the ABA signalling, JA signalling, proline biosynthesis and reactive oxygen species (ROS) scavenging processes were up-regulated in IbRAP2-12 overexpression lines under salt and drought stresses. In comparing with WT, the contents of ABA, JA and proline were significantly increased, while hydrogen peroxide (14202) and the rate of water loss were significantly reduced in transgenic lines under salt and drought stresses. All these results demonstrated the roles of IbRAP2-12 in enhancing salt and drought tolerance in transgenic Arabidopsis lines. Thus, this IbRAP2-12 gene can be used to increase the tolerance ability during abiotic stresses in plants.