Multiresidue analysis of pesticides in vegetables and fruits using two-layered column with graphitized carbon and water absorbent polymer
Multiresidue analysis of pesticides in vegetables and fruits using two-layered column with graphitized carbon and water absorbent polymer
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作者：
Obana, H;Akutsu, K;Hori, S
建立了非脂肪类蔬菜和水果中农药多残留的高通量分析方法。该方法包括一个单一的提取和一个单一的清理程序。食品样品用乙酸乙酯提取，提取液和食品渣滓的混合物直接倒入清理柱。净化柱由两层吸水聚合物（上层）和石墨化碳（下层）组成，它们被包装在一个储层（75毫升）的圆筒柱中。聚合物除去萃取物中的水分，而碳进行清理。在回收试验中，添加了110种农药，菠菜和橙子的平均回收率超过95%。5次试验中，大部分农药回收率在70 ~ 115%之间，RSD通常< 10%。从13份样品中提取12种农药进行残留分析。两种方法除芹菜中百菌清残留量较低外，其余两种方法的残留量基本一致。结果表明，该方法可用于食品中农药残留的监测。
A high-throughput multiresidue analysis of pesticides in non-fatty vegetables and fruits was developed. The method consisted of a single extraction and a single clean-up procedure. Food samples were extracted with ethyl acetate and the mixture of extract and food dregs were poured directly into the clean-up column. The clean-up column consisted of two layers of water-absorbent polymer (upper) and graphitized carbon (lower), which were packed in a reservoir (75 ml) of a cartridge column. The polymer removed water in the extract while the carbon performed clean-up. In a recovery test, 110 pesticides were spiked and average recoveries were more than 95% from spinach and orange. Most pesticides were recovered in the range 70-115% with RSD usually < 10% for five experiments. The residue analyses were performed by the extraction of 12 pesticides from 13 samples. The two methods resulted in similar residue levels except chlorothalonil in celery, for which the result was lower with the proposed method. The results confirmed that the proposed method could be applied to monitoring of pesticide residue in foods.