T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic stresses
T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic stresses
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DOI：
10.1007/s11103-007-9213-4
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发表时间：
2007-11-01
影响因子：
5.1
通讯作者：
Kim, Seong-Ryong
中科院分区：
文献类型：
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作者：
Koh, Serry;Lee, Sang-Choon;Kim, Seong-Ryong
在低温或盐胁迫条件下筛选t- dna标记的水稻植株，以确定其非生物胁迫反应的分子机制。第0-165-65行被确定为盐响应行。通过反相PCR发现，导致这种gus阳性表型的基因为OsGSK1 ((O)位于bar ryza (s)位于bar active (g)位于bar lycogen （s)位于bar inase3样基因1下方的bar ynthase (k)位于bar inase3样基因1下方），是植物GSK3/ shaggy样蛋白激酶基因的成员，是bar rasassino甾体i (n)位于bar sensitive 2 （BIN2）下方的拟南芥（AtSK21）的同源基因。Northern blot分析显示，OsGSK1在发育中的穗中表达量最高，表明其表达具有发育阶段特异性。与非转基因分离体（NT）相比，OsGSK1基因敲除（KO）突变体对冷、热、盐和干旱胁迫的耐受性增强。全长OsGSK1的过表达导致生长发育迟缓的表型与功能获得型BIN/AtSK21突变体相似。这表明OsGSK1可能是一个功能性的水稻同源物，作为油菜素内酯（BR）信号传导的负调节因子。因此，我们认为OsGSK1可能在胁迫信号转导途径和花发育过程中具有生理作用。
T-DNA-tagged rice plants were screened under cold- or salt-stress conditions to determine the genes involved in the molecular mechanism for their abiotic-stress response. Line 0-165-65 was identified as a salt-responsive line. The gene responsible for this GUS-positive phenotype was revealed by inverse PCR as OsGSK1 ((O) under bar ryza (s) under bar ativa (g) under bar lycogen (s) under bar ynthase (k) under bar inase3-like gene 1), a member of the plant GSK3/SHAGGY-like protein kinase genes and an orthologue of the Arabidopsis (b) under bar rassinosteroid i (n) under bar sensitive 2 (BIN2), AtSK21. Northern blot analysis showed that OsGSK1 was most highly detected in the developing panicles, suggesting that its expression is developmental stage specific. Knockout (KO) mutants of OsGSK1 showed enhanced tolerance to cold, heat, salt, and drought stresses when compared with non-transgenic segregants (NT). Overexpression of the full-length OsGSK1 led to a stunted growth phenotype similar to the one observed with the gain-of-function BIN/AtSK21 mutant. This suggests that OsGSK1 might be a functional rice orthologue that serves as a negative regulator of brassinosteroid (BR)-signaling. Therefore, we propose that stress-responsive OsGSK1 may have physiological roles in stress signal-transduction pathways and floral developmental processes.