Identification and molecular characterization of viruses infecting cucurbits in Pakistan
Identification and molecular characterization of viruses infecting cucurbits in Pakistan
复制标题
DOI：
10.1111/j.1439-0434.2004.00915.x
复制
发表时间：
2004-12-01
影响因子：
1.5
通讯作者：
Okuda, S
中科院分区：
文献类型：
--
作者：
Ali, A;Natsuaki, T;Okuda, S
南瓜作为夏季和冬季作物在巴基斯坦西北边境省种植。在调查期间，大部分瓜类蔬菜田出现花叶、斑驳、褪绿和叶片扭曲等症状。用斑点免疫结合试验检测到黄瓜绿色斑驳花叶病毒（CGMMV）、小西葫芦黄化花叶病毒（ZYMV）、西瓜花叶病毒（WMV）和番木瓜环斑病毒（PRSV）侵染瓜类植物。CGMMV分布广泛，感染率为46.9%，其次是ZYMV（14.8%）、WMV（12.5%）和PRSV（7.8%）。多重感染很常见，42%的样本感染了两种病毒，8%感染了三种病毒。测定了这4个病毒的外壳蛋白（CP）基因的核苷酸序列，推导的氨基酸序列比较表明，ZYMV-Pak分离物与世界上已报道的ZYMV其它分离物的相似性为88.3-99%。WMV、CGMMV和PRSV巴基斯坦分离株的氨基酸序列同源性分别为96.8%~ 98.4%、98.1%~ 99.4%和79.3%~ 84.2%。WMV和CGMMV的序列变异性很小。ZYMV-Pak与美国分离物的亲缘关系较近，PRSV-Pak与PRSV印度分离物的亲缘关系较近，可能反映了这些分离物之间的地理关系。
Cucurbits are grown throughout the North-West Frontier Province of Pakistan as summer and winter crops. Plants having mosaic, mottling, chlorosis and leaf distortion symptoms were frequently found in most of the cucurbit fields during the survey. Using dot immunobinding assay, Cucumber green mottle mosaic virus (CGMMV), Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV) and Papaya ringspot virus (PRSV) were found infecting cucurbits. CGMMV was widespread, infecting 46.9% of the samples tested followed by ZYMV (14.8%), WMV (12.5%) and PRSV (7.8%). Multiple infections were common with 42% of the samples being infected with two viruses and 8% with three viruses. The nucleotide sequences of the coat protein (CP) genes of these four viruses were determined and deduced amino acid sequence comparisons revealed 88.3-99% similarity of the ZYMV-Pak isolate with other isolates of ZYMV reported worldwide. The amino acid sequence identity of Pakistani isolates of WMV, CGMMV and PRSV ranged from 96.8 to 98.4%, 98.1 to 99.4% and 79.3 to 84.2%, respectively, with other isolates reported elsewhere. Little variability was observed in the sequences of WMV and CGMMV. ZYMV-Pak was very close to the USA isolate, and the PRSV-Pak isolate was close to Indian isolates of PRSV possibly reflecting the geographical relationship between these isolates.