The rat androgen receptor: primary structure, autoregulation of its messenger ribonucleic acid, and immunocytochemical localization of the receptor protein.
The rat androgen receptor: primary structure, autoregulation of its messenger ribonucleic acid, and immunocytochemical localization of the receptor protein.
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DOI：
10.1210/mend-2-12-1276
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发表时间：
1988-12
影响因子：
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通讯作者：
Jiann-an Tan;D. R. Joseph;V. Quarmby;D. Lubahn;M. Sar;F. S. French;E. M. Wilson
中科院分区：
文献类型：
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作者：
Jiann-an Tan;D. R. Joseph;V. Quarmby;D. Lubahn;M. Sar;F. S. French;E. M. Wilson
从大鼠附睾噬菌体λ gt 11文库中分离的三个cDNA克隆中确定了一个长度为4，181个碱基对的复合雄激素受体DNA序列。902个氨基酸的开放阅读框编码98，227 mol wt.类固醇受体家族的结构域特征包括具有五个重复氨基酸基序的氨基末端区域、与其它类固醇受体同源的中心DNA结合域和羧基末端类固醇结合区域。北方印迹分析中使用的受体cDNA探针与大鼠雄性生殖组织中占优势的10-脱氢酶雄激素受体mRNA杂交。雄激素受体mRNA的自动调节表明，在大鼠腹侧前列腺的水平增加，10-脱氢酶mRNA去势后和雄激素再刺激后的受体mRNA的抑制。合成了一段15个氨基酸的雄激素受体肽段，并将其作为免疫原在家兔体内诱导受体抗体。通过酶联免疫吸附试验和对本地[3 H]二氢睾酮标记的雄激素受体的高滴度的抗血清反应，证明了蔗糖梯度离心测定的受体沉降率的增加。免疫细胞化学染色显示雄激素受体定位于大鼠前列腺腹侧上皮细胞核。
A composite androgen receptor DNA sequence 4,181 base pairs in length was determined from three cDNA clones isolated from a rat epididymal bacteriophage lambda gt11 library. An open reading frame of 902 amino acids encodes a protein of 98,227 mol wt. Structural domains characteristic of the steroid receptor family include an amino-terminal region with five repeated amino acid motifs, a central DNA-binding domain homologous with other steroid receptors, and a carboxyl-terminal steroid-binding region. A receptor cDNA probe used in Northern blot analysis hybridized with a predominant 10-kilobase androgen receptor mRNA in male reproductive tissues of the rat. Autoregulation of androgen receptor mRNA was indicated in rat ventral prostate by an increase in the level of 10-kilobase mRNA after castration and suppression of receptor mRNA upon androgen restimulation. A 15 amino acid peptide with sequence derived from the deduced androgen receptor sequence was synthesized and used as immunogen in raising receptor antibodies in rabbits. Antisera reacted with high titer against the synthetic peptide by enzyme-linked immunosorbent assay and against the native [3H]dihydrotestosterone-labeled androgen receptor as evidenced by an increase in receptor sedimentation rate determined by sucrose gradient centrifugation. Immunocytochemical staining localized the androgen receptor to epithelial cell nuclei in rat ventral prostate.