Influences of octopamine and juvenile hormone on locomotor behavior and period gene expression in the honeybee, Apis mellifera
Influences of octopamine and juvenile hormone on locomotor behavior and period gene expression in the honeybee, Apis mellifera
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DOI：
10.1007/s00359-006-0179-5
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发表时间：
2007-02-01
影响因子：
2.1
通讯作者：
Meshi, Avital
中科院分区：
文献类型：
--
作者：
Bloch, Guy;Meshi, Avital
八胺(OA)和保幼激素(JH)参与了蜜蜂基于年龄的劳动分工的调节。我们测试了这两个神经内分泌信号在昼夜节律和时钟基因周期(PER)的大脑表达中影响任务相关可塑性的假设。使用OA、OA拮抗剂(依匹斯汀)或两者同时使用，并不影响昼夜节律开始时的年龄或在持续黑暗中的自由跑动期(DD)。幼蜂在明暗(LD)光照条件下口服OA 6d后再经DD处理，在LD光照的前4天和DD的前4天，阿尔法(每日活动开始和抵消之间的时间)降低。口服OA、依匹斯汀或两者同时使用，但不控制JH水平，会导致年轻蜜蜂的平均每日水平增加，并导致大脑Per mRNA振荡的异常模式。这些结果表明，OA和JH不影响中央起搏器的发育或功能，而是影响大脑中时钟基因的表达和运动行为的特征，这些被认为不受昼夜节律起搏器的直接控制。
Octopamine (OA) and juvenile hormone (JH) are implicated in the regulation of age-based division of labor in the honeybee, Apis mellifera. We tested the hypothesis that these two neuroendocrine signals influence task-associated plasticity in circadian and diurnal rhythms, and in brain expression of the clock gene period (per). Treatment with OA, OA antagonist (epinastine), or both, did not affect the age at onset of circadian rhythmicity or the free running period in constant darkness (DD). Young bees orally treated with OA in light-dark (LD) illumination regime for 6 days followed by DD showed reduced alpha (the period between the daily onset and offset of activity) during the first 4 days in LD and the first 4 days in DD. Oral treatment with OA, epinastine, or both, but not manipulations of JH levels, caused increased average daily levels and aberrant patterns of brain per mRNA oscillation in young bees. These results suggest that OA and JH do not influence the development or function of the central pacemaker but rather that OA influences the brain expression of a clock gene and characteristics of locomotor behavior that are not thought to be under direct control of the circadian pacemaker.