The removal of the sperm perinuclear theca and its association with the bovine oocyte surface during fertilization.
The removal of the sperm perinuclear theca and its association with the bovine oocyte surface during fertilization.
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受精过程中精子核周卵泡膜的去除及其与牛卵母细胞表面的关联。
DOI：
10.1006/dbio.1997.8618
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Schatten,G
中科院分区：
文献类型：
--
作者：
Sutovsky,P;Oko,R;Hewitson,L;Schatten,G
核周膜（PT）是一种独特的细胞骨架结构，其前部插入哺乳动物精子头部的顶体内膜和核膜之间，并且对于精子发生和精子结构的稳定化是重要的（Oko和Maravei，Biol.Reprod.50，1000-1014，1994; Oko和Maravei，Microsc. Res.Tech.32，520-532，1995）。利用免疫荧光标记的受精牛卵母细胞和连续切片的超微结构分析，我们证明，PT从精子细胞核的损失后，精子质膜和卵母细胞皮质与PT的相互作用。这些事件先于雄性原核的发育。PT的去除涉及到富含肌动蛋白微丝的细长卵母细胞微绒毛，因为它可以被破坏微绒毛的药物细胞松弛素B阻断。二硫键的还原，这是一个主要因素，支持哺乳动物受精过程中的精子核和附属结构的解体，似乎对PT在体外的影响不大，证明了与二硫键还原剂dithiothreitol.In体内，完整的公牛精子显微注射到成熟的卵母细胞中的处理的分离公牛精子不经历解体的PT。因此，精子核的解凝聚不会发生。这些数据表明，PT的卵母细胞微绒毛区域的结合，并从精子核中去除构成了哺乳动物受精的早期步骤，这是所需的精子核转化为男性原核。
The perinuclear theca (PT) is a unique cytoskeletal structure whose anterior part is intercalated between the inner acrosomal membrane and the nuclear envelope of the mammalian sperm head and is important for spermiogenesis and stabilization of sperm structures (Oko and Maravei,Biol. Reprod.50, 1000–1014, 1994; Oko and Maravei,Microsc. Res. Tech.32, 520–532, 1995). Using immunofluorescence labeling of inseminated bovine oocytes and serial sectioning–ultrastructural analysis, we demonstrate that the PT is removed from the sperm nucleus following the loss of the sperm plasma membrane and the interaction of oocyte cortex with the PT. These events precede the development of the male pronucleus. The removal of the PT involves the elongated oocyte microvilli, rich in actin microfilaments, since it can be blocked by the microfilament-disrupting drug cytochalasin B. Reduction of disulfide bonds, which is a major factor supporting the disassembly of the sperm nucleus and accessory structures during mammalian fertilization, seems to exert little effect on the PTin vitro,as evidenced by the treatment of isolated bull sperm with the disulfide bond-reducing agent dithiothreitol.In vivo,intact bull sperm microinjected into mature oocytes do not undergo disassembly of the PT. Consequently, the decondensation of the sperm nucleus does not occur. These data suggest that the binding of the PT to the oocyte microvillar region and its removal from the sperm nucleus constitute an early step in mammalian fertilization, which is required for the conversion of the sperm nucleus into a male pronucleus.
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