Nicotiana cybrids with Petunia chloroplasts
Nicotiana cybrids with Petunia chloroplasts
复制标题
具有矮牵牛叶绿体的烟草杂种
DOI：
10.1007/bf00266547
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发表时间：
1986
影响因子：
5.4
通讯作者：
H. Bonnett
中科院分区：
文献类型：
--
作者：
K. Glimelius;H. Bonnett
用叶绿体缺陷型烟草品种的原生质体与辐照过的矮牵牛原生质体进行融合。再生了100多株光合自养植物，其中94株进行了Petuniachloroplast性状的测试，除一株外，其余所有植株都具有Petuniachloroplast，这是基于它们对真菌毒素tentoxin的敏感性。对3株敏感植株的叶绿体DNA进行了分析，结果表明与Petuniachloroplast DNA完全相同。大部分植株（约70%）的氮素水平正常。tabacumplants，基于形态学和染色体数目。它们是完全可育的，具有正常的花粉生活力、结实率和种子生活力。其余30%的植物表现出不同程度的营养和生殖异常。
Protoplasts of a chloroplast-defective cultivar ofNicotianatabacum were fused with gamma-irradiated protoplasts ofPetunia hybrida. Over 100 photoautotrophic plants were regenerated; of these 94 were tested forPetuniachloroplast traits and all but one hadPetuniachloroplasts based on their sensitivity to the fungal toxin, tentoxin. Chloroplast DNA was analysed for 3 of the sensitive plants and was shown to be identical toPetuniachloroplast DNA. Most of the plants (about 70%) appeared to be normalN. tabacumplants, based on morphology and chromosome number. They were fully fertile with normal pollen viability, seed set, and seed viability. The remaining 30% of the plants showed varying degrees of vegetative and reproductive abnormalities.