EFFECTS OF CRYOPRESERVATION ON HUMAN SPERM ACROSOMES
EFFECTS OF CRYOPRESERVATION ON HUMAN SPERM ACROSOMES
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DOI：
10.1093/oxfordjournals.humrep.a137526
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发表时间：
1991-10-01
影响因子：
6.1
通讯作者：
HANKS, SE
中科院分区：
文献类型：
--
作者：
CROSS, NL;HANKS, SE
测定人精子冷冻前后顶蛋白总活性和顶体状态。使用了三种不同的低温保存方案。在低温保存期间，顶蛋白活性和完整顶体的发生率均下降。顶体缺失导致了顶蛋白活性的降低（r = 0.29, P < 0.05）。三种低温保存方案的效果无显著差异。精子活力下降幅度（平均43%）大于顶体完整精子百分比（27%）和顶蛋白总活性（24%）。这些测量表明顶体损伤可能继发于细胞死亡。通过确定冷冻保存后精子顶体的状态验证了这一假设。解冻后仍能活动的精子平均96%顶体完好；然而，它们的顶蛋白活性明显低于运动的未冷冻精子。这些观察结果支持了冻存导致顶体丢失与细胞死亡相关的观点，但也证明了冻存后顶体完整精子的顶蛋白总活性降低。
Total acrosin activity and acrosomal status were determined before and after cryopreserving human spermatozoa. Three different cryopreservation protocols were used. Both acrosin activity and the incidence of intact acrosomes decreased during cryopreservation. The magnitudes of the decreases were weakly but significantly correlated (r = 0.29, P < 0.05), suggesting that acrosomal loss contributed to the decrease in acrosin activity. The effects of the three cryopreservation protocols were not significantly different. Motility decreased more (average 43%) than did the percentage of spermatozoa with intact acrosomes (27%) and the total acrosin activity (24%). These measurements suggested that acrosomal damage may have been secondary to cell death. This hypothesis was tested by determining the acrosomal status of spermatozoa that survived cryopreservation. Spermatozoa that were motile after thawing averaged 96% acrosome-intact; their acrosin activity, however, was significantly less than that of motile, unfrozen spermatozoa. These observations support the idea that the acrosomal loss due to cryopreservation is associated with cell death but also demonstrate decreased total acrosin activity of the acrosome-intact spermatozoa that survive cryopreservation.