Detection and assessment of the antibiotic resistance of Enterobacteriaceae recovered from bioaerosols in the Choqueyapu River area, La Paz – Bolivia
Detection and assessment of the antibiotic resistance of Enterobacteriaceae recovered from bioaerosols in the Choqueyapu River area, La Paz – Bolivia
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玻利维亚拉巴斯 Choqueyapu 河地区生物气溶胶中肠杆菌科抗生素耐药性的检测和评估
DOI：
10.1016/j.scitotenv.2020.143340
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发表时间：
2021
影响因子：
9.8
通讯作者：
Herrera, Jhoana
中科院分区：
文献类型：
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作者：
Medina, Claudia;Ginn, Olivia;Brown, Joe;Soria, Freddy;Garvizu, Carolina;Salazar, Ángela;Tancara, Alejandra;Herrera, Jhoana
乔克亚普河流经人口稠密的城市地区，是一条污染严重的水道，与乔克亚普河有关的微生物污染以及拉巴斯市没有废水处理厂威胁着公众健康。我们用撞击法收集了这条河附近的空气样本。实验室分析确定了肠杆菌科的存在，报告采样空气的最大浓度为86.11 CFU/m3。通过纸片扩散法检测阳性样本对阿莫西林-克拉维酸、环丙沙星、庆大霉素、美罗培南、磺胺甲恶唑-甲氧苄啶和四环素的抗生素敏感性。四环素（50%的分离株）和磺胺甲恶唑-甲氧苄啶（38.9%）的抗生素耐药率最高，而美罗培南的耐药率最低（5.6%）。试点研究结果的比较[Chavez，2019和Salazar等人在2018年雨季详细阐述，2020年]，目前的研究已经完成，强调季节性影响空气中肠杆菌属浓度。此外，还确定了四环素、庆大霉素和环丙沙星的抗生素耐药性增加;磺胺甲恶唑-甲氧苄啶、美罗培南和阿莫西林-克拉维酸的耐药性减少。
As a highly contaminated waterway flowing through a densely populated urban area, microbiological pollution associated with the Choqueyapu River and the absence of a wastewater treatment plant in La Paz city threatens public health. We collected air samples adjacent to this river using impingement. Laboratory analyses identified the presence ofEnterobacteriaceae, reporting a maximum concentration of 86,11 CFU/m3of sampled air. Positive samples were tested for antibiotic susceptibility against the antibiotics amoxicillin-clavulanic acid, ciprofloxacin, gentamicin, meropenem, sulfamethoxazole-trimethoprim and tetracycline via disk diffusion. The highest percentages of antibiotic resistance were registered for tetracycline (50% of isolates) and sulfamethoxazole-trimethoprim (38,9%), while the lowest resistance profile was reported for meropenem (5,6%). A comparison of results obtained on the pilot studies [elaborated during the wet season of 2018 by Chavez, 2019 and Salazar et al., 2020] and the present study has been done, highlighting seasonal effects over airborneEnterobacteriaceaeconcentration. Also, it was determined an increase of antibiotic resistance for tetracycline, gentamicin and ciprofloxacin; and a reduction for sulfamethoxazole-trimethoprim, meropenem and amoxicillin-clavulanic acid.