A simple method for human peripheral blood monocyte isolation
A simple method for human peripheral blood monocyte isolation
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DOI:
10.1590/s0074-02762000000200014
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发表时间:
2000-03-01
影响因子:
2.8
通讯作者:
Netto, MB
中科院分区:
文献类型:
--
作者:
de Almeida, MC;Silva, AC;Netto, MB
We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy, coat cells. The use of simple laboratory equipment and a relatively cheap I reagent makes the described method a convenient approach to obtaining human monocytes.