Identification of 3′-hydroxygenistein as a potent melanogenesis inhibitor from biotransformation of genistein by recombinant Pichia pastoris
Identification of 3′-hydroxygenistein as a potent melanogenesis inhibitor from biotransformation of genistein by recombinant Pichia pastoris
复制标题
DOI：
10.1016/j.procbio.2015.06.007
复制
发表时间：
2015-10-01
影响因子：
4.4
通讯作者：
Chang, Te-Sheng
中科院分区：
文献类型：
--
作者：
Ding, Hsiou-Yu;Chiang, Chien-Min;Chang, Te-Sheng
利用重组毕赤酵母对染料木素进行生物转化，分离得到一种产物，经质量分析、核磁共振氢谱和碳谱分析鉴定为3‘-羟基染料木素。在51发酵罐中生物转化的最大产物浓度和转化率分别为3.5 mg/l和14%。以蘑菇酪氨酸酶为模型，研究了3‘-羟基染料木素对酪氨酸酶活性的抑制作用。结果表明，3‘-羟基染料木素对酪氨酸酶活性有较强的抑制作用，IC50值为15.9 mU M。此外，还观察了3’-羟基染料木素对体外培养的B16黑色素瘤细胞黑素合成的抑制作用，结果表明，3‘-羟基染料木素在无毒浓度范围内呈剂量依赖性抑制黑素生成。综上所述，重组酵母由金雀异黄素产生的3‘-羟基染料木素被证实是一种有效的酪氨酸酶抑制剂，并能抑制B16细胞的黑素合成。(C)2015爱思唯尔有限公司。保留所有权利。
A product resulting from the biotransformation of genistein by a recombinant Pichia pastoris was isolated and identified as 3'-hydroxygenistein, on the basis of mass, H-1 NMR, and C-13 NMR spectrophotometric analysis. The maximal product concentration and the conversion yield of the biotransformation in a 51 fermenter were 3.5 mg/1 and 14%, respectively. The inhibitory effects of 3'-hydroxygenistein on tyrosinase activity were investigated in vitro using mushroom tyrosinase. The results showed that 3'-hydroxygenistein potently inhibited tyrosinase activity with an IC50 value of 15.9 mu M. Furthermore, the inhibitory effects of 3'-hydroxygenistein on melanogenesis were also investigated in vitro in cultured B16 melanoma cells, and it was shown that 3'-hydroxygenistein dose-dependently inhibited melanogenesis in non-toxic concentrations. In summary, the 3'-hydroxygenistein that was produced from genistein by the recombinant yeast was confirmed as a potent tyrosinase inhibitor and inhibited melanogenesis in B16 cells. (C) 2015 Elsevier Ltd. All rights reserved.