Effects of catecholaminergic nerve lesion on endometrial development during early pregnancy in Mice.
Effects of catecholaminergic nerve lesion on endometrial development during early pregnancy in Mice.
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DOI：
10.14670/hh-11-684
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发表时间：
2016-04
影响因子：
2
通讯作者：
Yulan Dong;Guanhui Liu;Zixu Wang;Jing Li;Jing Cao;Yaoxing Chen
中科院分区：
文献类型：
--
作者：
Yulan Dong;Guanhui Liu;Zixu Wang;Jing Li;Jing Cao;Yaoxing Chen
在怀孕和产后期间，母亲的压力是常见的。这种压力通常会激活交感神经系统，从而释放儿茶酚胺。本研究探讨神经毒素6-羟多巴胺（6-OHDA）切除交感神经对胚胎着床的影响，探讨交感神经切除对妊娠早期子宫内膜结构重建的影响机制。6- ohda处理小鼠妊娠早期子宫内膜内子宫腺发育不良，腺体上皮排列不规则。部分腺体上皮可见空泡、核固缩和浆胞质增生。治疗组妊娠5-9 d （E5-9）子宫腺百分率和增殖细胞核抗原（PCNA）阳性密度显著降低，Fas配体（FasL）表达降低。6-OHDA组小鼠子宫内抗氧化酶活性低于对照组小鼠，而丙二醛（MDA）水平高于对照组小鼠。同样，6-OHDA处理后，妊娠早期诱导型一氧化氮合酶（iNOS）阳性细胞数量显著增加。我们的研究结果表明，6-OHDA诱导的外周儿茶酚胺能神经病变通过破坏子宫内膜腺发育，增加子宫内膜氧化应激和iNOS表达，导致不良妊娠结局。因此，儿茶酚胺能神经可能通过氧化状态调节和子宫内膜腺重建对妊娠早期胚泡着床、胎儿存活和发育产生有利影响。
Maternal stress is common during pregnancy and the postnatal period. This stress typically activates the sympathetic nervous system which releases catecholamines. This study explored the influence of sympathectomy by using neurotoxin 6-hydroxydopamine (6-OHDA) on embryo implantation, and investigated the influence mechanism of sympathectomy on reconstruction of endometrial structure during early pregnancy. In the 6-OHDA-treated mice, uterine glands in the endometrium developed poorly, and the gland epithelia were arranged irregularly during early pregnancy. Furthermore, vacuoles, karyopykosis and plasmarrhexis appeared in some gland epithelia. The percentage of uterine glands and the density of proliferating cell nuclear antigen (PCNA) positivity were dramatically decreased, and Fas ligand (FasL) expression was decreased in cells from pregnancy days 5-9 (E5-9) in the treated group. Antioxidant enzyme activity levels in uteri were lower but the malondialdehyde (MDA) levels were higher in the 6-OHDA mice than those in the control mice at E5-9. Similarly, the number of inducible nitric oxide synthase (iNOS) positive cells was significantly increased during early pregnancy following treatment with 6-OHDA. Our results have indicated that peripheral catecholaminergic nerve lesions induced by 6-OHDA cause adverse pregnancy outcomes through disruption of endometrial gland development, which increases oxidative stress and iNOS expression in the endometrium. Thus, catecholaminergic nerves might favourably influence blastocyst implantation, foetal survival and development during early pregnancy by oxidative state regulation and endometrial gland reconstruction.